Cell Reports
Volume 12, Issue 2, 14 July 2015, Pages 300-312
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Article
Cooperative Transcriptional Activation of Antimicrobial Genes by STAT and NF-κB Pathways by Concerted Recruitment of the Mediator Complex

https://doi.org/10.1016/j.celrep.2015.06.021Get rights and content
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Highlights

  • STAT-NF-κB cooperativity shapes the transcriptional response to infection

  • NF-κB recruits RNA polymerase II kinase complexes and enzymes marking active chromatin

  • NF-κB attracts the mediator kinase module, whereas STATs contact the core mediator

  • STAT-NF-κB cooperation tethers a complete, functional mediator to antimicrobial genes

Summary

The transcriptional response to infection with the bacterium Listeria monocytogenes (Lm) requires cooperative signals of the type I interferon (IFN-I)-stimulated JAK-STAT and proinflammatory NF-κB pathways. Using ChIP-seq analysis, we define genes induced in Lm-infected macrophages through synergistic transcriptional activation by NF-κB and the IFN-I-activated transcription factor ISGF3. Using the Nos2 and IL6 genes as prime examples of this group, we show that NF-κB functions to recruit enzymes that establish histone marks of transcriptionally active genes. In addition, NF-κB regulates transcriptional elongation by employing the mediator kinase module for the recruitment of the pTEFb complex. ISGF3 has a major role in associating the core mediator with the transcription start as a prerequisite for TFIID and RNA polymerase II (Pol II) binding. Our data suggest that the functional cooperation between two major antimicrobial pathways is based on promoter priming by NF-κB and the engagement of the core mediator for Pol II binding by ISGF3.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).