CommunicationSensing carboxylesterase 1 in living systems by a practical and isoform-specific fluorescent probe
Graphical abstract
A practical and isoform-specific fluorescent probe based on BIDIPY dye have been designed, synthesized and well characterized for sensing CES1 activities in various biological systems such as living cells, tissues and zebrafish.
Section snippets
Acknowledgments
This work was supported by the National Natural Science Foundation of China (Nos. 21572029, 31600641, 81703604, 81773687, 81672961 and 81573501), the National Key Research and Development Program of China (Nos. 2017YFC1700200 and 2017YFC1702000), Program of Shanghai Academic/Technology Research Leader (No. 18XD1403600), Shuguang Program (No. 18SG40) supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission and the Innovative Entrepreneurship Program of
References (41)
- et al.
J. Mol. Biol.
(2006) - et al.
Acta Pharm. Sin. B
(2018) - et al.
Fitoterapia
(2017) - et al.
Chin. Chem. Lett.
(2017) - et al.
Anal. Chim. Acta
(2017) - et al.
Chin. Chem. Lett.
(2018) - et al.
Chin. Chem. Lett.
(2017) - et al.
Chin. Chem. Lett.
(2017) - et al.
Chin. Chem. Lett.
(2017) - et al.
Biosens. Bioelectron.
(2015)
Biosens. Bioelectron.
Eur. J. Pharm. Sci.
Biosens. Bioelectron.
J. Chromatogr. B
Life. Sci.
Biosens. Bioelectron.
Hepatology
Nat. Chem. Biol.
Curr. Med. Chem.
J. Biochem. Mol. Toxic.
Cited by (30)
Ratiometric fluorescent probe with signals from two near-infrared channels for esterase activity and its application in biological imaging
2023, Sensors and Actuators B: ChemicalDeciphering the species differences in CES1A-mediated hydrolytic metabolism by using a bioluminescence substrate
2022, Chemico-Biological InteractionsCitation Excerpt :Although the nucleotide and amino acid sequences for mammalian CES1A have been well investigated, the interspecies differences in CES1A function have not been well-investigated yet, which greatly limit explore of the potential role of CES1A in cellular processes and human diseases, and the discovery of CES1A inhibitors/inducers as therapeutic agents. Recent years, a number of optical substrates for CES1A have been developed, which convenient for sense endogenous CES1A function in biological system, and high-throughput screening and development of CES1A inhibitors/inducers [13,23–32]. The emergence of these tool molecules strongly facilitates the investigations to evaluate the interspecies differences of CES1A function, in terms of its enzymic kinetic parameters and the response behavior towards various inhibitors.
Explorations into the meso-substituted BODIPY-based fluorescent probes for biomedical sensing and imaging
2022, TrAC - Trends in Analytical ChemistryHigh-throughput optical assays for sensing serine hydrolases in living systems and their applications
2022, TrAC - Trends in Analytical ChemistryCitation Excerpt :This probe was successfully used for imaging CES1A in SKOV-3-Luc + cells. Later, we modified the structure of Bodipy, and designed several fluorescent substrates (CES1A-3 and CES1A-6) for CES1A, Caco-2 and A549 cell lines were used to validate the probes' ability to report on endogenous CES1A activity in living cells [40,110]. We also used molecular docking-based virtual screening combined with reaction phenotyping assays to construct a more robust bioluminescent substrate(s) for sensing CES1A in real samples [41].
- 1
These authors contributed equally to this work.