Elsevier

Brain Research

Volume 1021, Issue 1, 17 September 2004, Pages 132-139
Brain Research

Short communication
Region-specific alterations in insulin-like growth factor-I receptor in the central nervous system of nNOS knockout mice

https://doi.org/10.1016/j.brainres.2004.06.043Get rights and content

Abstract

In the present study, we investigated layer-specific changes in insulin-like growth factor-I (IGF-I) receptor localization in the cerebral cortex, hippocampus and cerebellum of neuronal nitric oxide synthase knockout (nNOS−/−) mice using immunohistochemistry. In the cerebral cortex of control mice, moderately stained cells were seen through the layers II–VI in several cortical regions. In nNOS−/− mice, there was a significant decrease in IGF-I receptor immunoreactivity in the same cortical regions. In the hippocampus of control mice, a distinct immunoreactivity pattern was observed in the CA1–3 areas and dentate gyrus. The immunoreactivity for IGF-I receptor was differentially decreased in each layer in nNOS−/− mice. In nNOS−/− cerebellum, IGF-I receptor immunoreactivity was also significantly decreased in each layer of cerebellar cortex and cerebellar nuclei. To clarify whether decreased expression of IGF-I receptor in nNOS−/− mice was specific, the expression of other receptors for IGF-I was also evaluated. Receptor tyrosine kinase type A (TrkA receptor) and TrkB receptor were differentially decreased in each layer of the hippocampus or cerebellum of nNOS−/− mice. Although further studies of functional features of IGF-I systems in the nNOS(−/−) mice are required, our first morphological data may provide insights into NO-induced changes in trophic support as well as basic knowledge required for the study of NO-associated neurological diseases.

Section snippets

Acknowledgements

This work was supported by grant No. R01-2003-000-10099-0 from the Basic Research Program of the Korea Science and Engineering Foundation. This study was supported in part by year 2004 BK21 project for Medicine, Dentistry and Pharmacy.

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