MiR-216a-5p/Hexokinase 2 axis regulates uveal melanoma growth through modulation of Warburg effect
Section snippets
Aerobic glycolysis is involved in the regulation of uveal melanoma cell proliferation by miR-216a-5p
Our previous work demonstrated the tumor suppressive role of miR-216a-5p, and since aerobic glycolysis plays a critical role in cancer proliferation, we investigated if aerobic glycolysis plays a role in the inhibition of uveal melanoma cell proliferation mediated by miR-216a-5p. Anti-miR-216a-5p to promotes proliferation in A375 and MUM-2B cells, and more importantly, the glycolytic inhibitor 2-deoxy-d-glucose (2-DG) suppressed the ability of anti-miR-216a-5p to promote proliferation in A375
Discussion
The reprogramming of cell metabolism is an emerging hallmark of cancer. In aerobic glycolysis (also known as the Warburg effect), tumor cells depend on glycolysis for energy even under normoxic conditions, thus providing them with a growth advantage. Targeting the process of glycolysis has been demonstrated to be an effective way in controlling tumor growth and enhancing anticancer therapies. During the process of glycolysis, metabolic enzymes have attracted the most attentions for targets of
Cell lines, plasmids, lentivirus, RNA oligonucleotides, reagents
Human uveal melanoma cell lines and human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (Manassas, VA, USA). Wild-type and mutated miR-216-5p putative targets on HK2 3′-UTR were cloned into pmiR-GLO dual-luciferase miRNA target expression vector (Promega). Lentiviral vectors expressing miR-216a or HK2 shRNA were constructed by cloning pre-miR-30a or HK2 short hairpin RNA fragments into pCDH and pSIH-H1-Puro (System Biosciences), respectively. The
Competing financial interest
The authors declare no competing financial interests.
Acknowledgements
This work was supported by National Natural Science Foundation (81770887, 61471039, 81471466, 81402345 and 81502264), Beijing Natural Science Foundation (7162180), Foundation in Education Ministry of Liaoning Province (JYTFUDF201749).
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