Biochemical and Biophysical Research Communications
Multipotent Adult Progenitor Cells (MAPC) contribute to hepatocarcinoma neovasculature☆
Section snippets
Materials and methods
Isolation and characterization of rMAPCs and cell lines. rMAPCs were obtained from the BM of Buffalo rats as previously described [15]. Briefly, BM was harvested by flushing the femurs and tibias of male Buffalo rats. Mononuclear cells were plated in MAPC expansion media [15]. Two days later, nonadherent cells were removed and adherent cells cultured for 2 more weeks. Only those wells where single clones were observed were expanded. After 3 or 4 weeks, cultures were depleted of CD45+/Ter119+
Isolation and characterization of rMAPCs
We have established five different new cell lines of rMAPCs from Buffalo rats using methods previously described with minor modifications. Cells were grown at low density (100–500 cells/cm2) and maintained for up to 190 population doubling (PD) with a doubling time of 36–48 hours. Cytogenetic analysis performed every 30 PDs showed a normal karyotype in each case (not shown). Based on cell size and cytoplasm complexity a population of small cells (4–6 μm) with reduced cytoplasm was observed (Fig. 1
Discussion
HCC is frequently diagnosed in advanced stages of the disease when there is no curative treatment available. Gene therapy has been used in these situations but despite promising preclinical data, (review in [19]) the results in patients have been rather modest [3]. The combination of gene therapy with cell therapy represents an attractive approach to enhance the antitumor effect of gene therapy-based strategies. For the treatment of tumors with high angiogenic activity such as HCC, a promising
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Supported in part by grants from the Government of Navarra, Spanish Ministerio de Ciencia y Tecnología (SAF 2002-04574-C02), Ministerio de Sanidad (PI050168), FEDER (INTERREG IIIA), PIUNA and the ‘‘UTE project CIMA’’.
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These authors contributed equally to this article and should be considered equal first authors.