Neuroprotection effects of retained acupuncture in neurotoxin-induced Parkinson’s disease mice
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► The results provide important information on integration of acupuncture into modern medicine and shed light into perspective the treatment of human Parkinson’s disease.
Introduction
Parkinson’s disease (PD) is one of the most common neurodegenerative disorders and affects about 1–2% of individuals over the age of 60 (Gasser, 2009). In Taiwan, it is estimated that the crude prevalence rate of idiopathic PD in persons aged 40 years and over is 706 per 100,000 individuals (Chen et al., 2009). PD is characterized by symptoms including rest tremors, postural instability, gait abnormality, bradykinesia and rigidity. The major pathological change of Parkinson’s disease is a progressive loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc), the loss of striatal dopaminergic fibers, a dramatic reduction in the striatal dopamine levels and the presence of neuronal proteinaceous aggregates called Lewy bodies (LBs) (Schober, 2004).
The exact etiology of PD, though extensively studied, remains unknown. A number of factors including oxidative stress, mitochondrial dysfunction, inflammation and apoptosis have been implicated in the pathogenesis of PD (Mounsey and Teismann, 2010). Accumulating evidence suggests that exposure to environmental toxins and genetic aberrance lead to dopaminergic neuronal loss and clinical Parkinsonism (Fahn, 2010, Liang et al., 2003, Przedborski et al., 2001). Current clinical therapy for PD is largely based on a dopamine replacement strategy, primarily by administration of the dopamine precursor levodopa; however, this is often associated with the development of levodopa-related motor complications (Stowe et al., 2010, Tomlinson et al., 2010). Many different treatment strategies such as genetic and implant therapies have been evaluated for neuroprotection and neuroprevention in the laboratory and in a clinical context (Fu et al., 2006, Poewe, 2009), but the outcome is far from satisfactory.
Since the 1990s the use of complementary and alternative medicine (CAM) worldwide has increased (Eisenberg et al., 1993). Therapies such as dietary supplements, vitamin therapy, exercise, physical therapy and massage therapy are reported to influence the symptoms of PD and/or the effectiveness of dopaminergic therapy (Zesiewicz and Evatt, 2009). Acupuncture therapy has been reported to show possible therapeutic effectiveness for PD in clinical trials, as manifested by an ameliorating of the clinical motor symptoms (Shulman et al., 2002) and an improvement in the quality of daily living (Zhuang and Wang, 2000); in addition acupuncture has no known interactions with other medication (Eng et al., 2006).
Previously, using functional magnetic resonance imaging (fMRI) and single photon emission computed tomography (SPECT), acupuncture analgesia has been shown to be a consequence of central modulation of the descending inhibitory pathway associated with pain sensation (Hsieh et al., 2001, Li et al., 2010, Wu et al., 2002). In human studies, activation of the hypothalamus has been identified as a cardinal finding of De-qi (Hsieh et al., 2001). Moreover, a recent fMRI study has suggested there is a cerebrocerebellar interaction during peripheral stimulation (Shih et al., 2009) and activation of the cerebellum can be visualized as occurring simultaneously with acupuncture treatment (Hsieh et al., 2001). This is indicative of an effect on the activation of extra-pyramidal system, an important area where the pathogenesis of PD occurs. Accordingly, it was our aim to investigate the effect of acupuncture on neurotoxin-induced PD mice.
Section snippets
Animals
Male C57BL/6 mice (National Laboratory Animal Center, Taiwan), 8 wk old, weighing 20–25 g, were housed in a temperature-controlled room under a 12-h light/12-h dark cycle (lights off from 8 p.m. to 8 a.m.). Food and water were provided ad libitum at all times. They were allowed to acclimate for at least 5 days before neurotoxin injection and were randomly assigned to different manipulation groups described below. The animals were treated under standardized conditions in accordance with “Guide for
The MPTP induced neurological deficit and the decreased striatal dopamine level occur in a dose-dependent manner
To validate the neurotoxin-induced PD model, various doses of MPTP (0, 7.5, 15, 30 mg/kg/d) were injected intraperitoneally into the mice for five consecutive days. A functional study using the pole test showed that there was a dose-dependent increase in the neurological deficit, (in terms of time of landing) of the MPTP-induced PD mice (Fig. 3A). By immunohistochemical analysis, there was a decrease in the TH (+) neurons, both qualitatively (Fig. 3B) and quantitatively (3C) in the MPTP-induced
Discussion
It is well known that treatments for Parkinson’s disease (PD) are not satisfactory. In this study we have demonstrated that retained acupuncture (RA) but not electroacupuncture (EA) attenuates MPTP-induced neuronal damage in mice and have verified these results using SPECT studies of the mice.
An animal model is an important tool when studying pathogenic mechanisms and therapeutic strategies in PD. Many in vivo models of PD have developed in a variety of species including rodents, dogs, pigs and
Acknowledgments
We declare that we have no conflicts of interest. This work was supported by grants from National Council Science (NSC 97-2627-B-010-008-).
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