Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume 1813, Issue 8, August 2011, Pages 1543-1553
Phosphorylated c-Jun and Fra-1 induce matrix metalloproteinase-1 and thereby regulate invasion activity of 143B osteosarcoma cells
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Graphical abstract
Highlights
► Osteosarcoma patients develop pulmonary metastases, but its mechanisms remain unclear. ► MMP-1 and CXCR4 were notably expressed in a highly metastatic osteosarcoma cell line. ► MAPK pathways activated AP-1 (c-Jun/Fra-1), and thereby regulated MMP-1 expression. ► The MAPK/AP-1/MMP-1 axis plays a central role in osteosarcoma invasion.
Abbreviations
AP-1
activator protein-1
CXCR4
C–X–C chemokine receptor type 4
ECM
extracellular matrix
ELISA
enzyme-linked immunosorbent assay
EMSA
electrophoretic mobility shift assay
ERK
extracellular signal-regulated kinase
ETS
E-twenty six
FCS
fetal calf serum
Fra-1
Fos-related antigen-1
GAPDH
glyceraldehyde 3-phosphate dehydrogenase
IL
interleukin
IL-2Rα
IL-2 receptor α chain
JNK
c-Jun NH2-terminal kinase
MAPK
mitogen-activated protein kinase
MMP
matrix metalloproteinase
MNNG
N-methyl-N′-nitro-N-nitrosoguanidine
NF-κB
nuclear factor-κB
PBS
phosphate-buffered saline
PEA3
polyomavirus enhancer-A binding protein-3
Rb
retinoblastoma protein
RT-PCR
reverse transcription-PCR
SD
standard deviation
SDF-1α
stromal cell-derived factor-1α
siRNA
small interfering RNA
WST-8
water-soluble tetrazolium-8
WT
wild type
Keywords
MMP-1
Osteosarcoma
Invasion
Pulmonary metastasis
c-Jun
Fra-1
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