Changes in neurodegeneration-related miRNAs in brains from CAPN1−/− mice
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Graphical abstract
Schematic representation of the effects of calpain-1 down-regulation on miRNA generation. Left. The activation of Dicer by calpain-1-mediated cleavage contributes to miRNA synthesis. Levels of active Dicer and of several miRNAs are decreased in brain of calpain-1 KO mice. Incubation of brain homogenates from calpain-1 KO mice with calpain-1 restores active Dicer and enhances miRNAs synthesis. Red fonts represent miRNAs which were validated by RT-qPCR.
Keywords
Calpain-1
Brain
Dicer
miRNA
Neurodegeneration
Abbreviations
DE miRNA
differentially expressed miRNAs
WT
wild-type
KO
knock-out
CPM
count-per-million
DEG
differentially expressed genes
RISC
RNA-induced silencing complex
TF
transcription factors
KEGG
Kyoto Encyclopedia of Genes and Genomes
PCA
principal component analysis
PC1
principal component 1
PC2
principal component 2
AD
Alzheimer's disease
PD
Parkinson's disease
HD
Huntington's disease
ALS
Amyotrophic lateral sclerosis
RT-qPCR
quantitative reverse transcription PCR
3’ UTR
3’ untranslated region
5’ UTR
5’ untranslated region
SNPs
single-nucleotide polymorphisms
poly-miRTSs
polymorphisms/mutations in miRNA target sites
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© 2021 The Author(s). Published by Elsevier B.V.