Elsevier

Aquaculture

Volumes 408–409, 15 September 2013, Pages 78-87
Aquaculture

Growing reared spider crabs (Maja squinado) to sexual maturity: The first empirical data and a predictive growth model

https://doi.org/10.1016/j.aquaculture.2013.05.031Get rights and content

Highlights

  • We report the first attempt to grow Maja squinado in captivity to sexual maturity.

  • The full life cycle of the Mediterranean spider crab was completed in captivity.

  • A growth model was developed to predict the molting probability and increment.

  • The size at which half of the females are mature was estimated at 103.6 mm.

  • The model will be useful for managing the stocking program for M. squinado.

Abstract

In this paper, we report the first successful attempt to grow Maja squinado in captivity from larvae to sexual maturity. Eggs were obtained from wild-caught crabs in the laboratory. The larvae–juveniles were reared together to an age of 150–210 days. Subsequently, up to seven consecutive molts were individually monitored to an age of 490 days maximum. Based on these individually monitored crabs, a growth model was developed to predict the molting probability and molting increment as a function of sex, temperature, and pre-molt size. The predictions of the model show that the females (25–75% percentiles of the 7th monitored molt: 106 to 139 mm and 456 to 654 days) appear to have a lower but less variable growth rate than the males (98 to 152 mm and 378 to 518 days). The size at first maturity for females was estimated to be 103.6 mm. Although the model was parameterized using individuals raised in aquaculture, data and models of this type are very scarce for crustaceans and will be useful for managing the ongoing stocking program for M. squinado in the Balearic Islands. In addition, the full life cycle of the Mediterranean spider crab was completed in captivity. The mating of laboratory-reared crabs was repeatedly observed, and viable eggs and larvae were obtained from laboratory-reared adults.

Introduction

Spider crab fisheries are well developed in many European countries, with annual catches of 6299 tonnes according to the FAO (2010). The Mediterranean spider crab Maja squinado is one of the largest and most valued European species. This species was abundant 50 years ago in the Spanish Mediterranean. Currently, it is scarce (Abad, 2003) and considered endangered (UNEP, 1996) or even extinct (e.g., Mallorca Island; García, 2007).

Environmental degradation and inappropriate fishery management are the most common causes of the decline and even the collapse of certain fisheries (Born et al., 2004). Stocking (releasing cultured individuals) is one of the conventional options for allowing depleted stocks to recover and for managing fisheries (Blankenship and Leber, 1995). However, stocking has been criticized because it may be neither effective nor economically viable. The limited usefulness of stocking has been attributed to the tendency of cultured marine species to show lower survival, growth rate, reproductive fitness, and genetic diversity than wild individuals (Araki and Schmid, 2010, Bartley, 1996). Additional adverse consequences of releasing cultured individuals include ecological competition with wild individuals, genetic introgression or the spread of diseases (Bell et al., 2008, Frankham et al., 2002, Kostow, 2009, Lorenzen et al., 2012). However, positive effects have also been widely demonstrated, such as the enhancement of population abundance after stocking (Blanco Gonzalez et al., 2008a, Blanco Gonzalez et al., 2008b). At least two species of crabs have shown some stock enhancement after stocking: the swimming crab Portunus trituberculatus (Ariyama, 2001, Hamasaki et al., 2011) and the blue crab Callinectes sapidus (Zohar et al., 2008). It has also been reported that in some cases released hatchery-reared individuals do not harm wild stocks (Blanco González and Umino, 2009, Blanco Gonzalez et al., 2008a, Blanco Gonzalez et al., 2008b, Jeong et al., 2007). In addition, certain restocking programs, such as that for the Japanese flounder, have been proven economically viable (Kitada, 1999, Kitada and Kishino, 2006). Moreover, restocking programs have been proven useful for preserving endangered species, such as the beluga sturgeon (Abdolhay, 2004, Ivanov et al., 1997).

The Mediterranean spider crab may be a candidate for a stock enhancement program (Durán et al., 2012). However, a number of preliminary studies must be completed prior to launching such a program to circumvent the potential problems listed above. Specifically, to allow a sustainable population to recover, the survival probability must be sufficiently large to allow some of the released crabs to reach sexual maturity and to reproduce in the wild. Thus, the accurate knowledge of the growth rate and of the size and age at sexual maturity is very important. Unfortunately, despite the increasing interest in developing growth models and in determining the factors affecting growth and sexual maturation, crustacean growth is a very challenging topic (Chen and Kennelly, 1999, Verdoit et al., 1999). Crustaceans lack a structure comparable to the otolith in fish, and the molting process precludes long-term marking. Therefore, growth is only well known for a few species, including the Alaskan king crab Paralithodes camtschatica (McCaughran and Powel, 1977), the American lobster Homarus americanus (Fogarty and Idoine, 1988), the Dungeness crab Cancer magister (Wainwright and Armstrong, 1993) and the blue crab C. sapidus (Brylawski and Miller, 2006). The patterns of growth are not well known for M. squinado. For this commercially important and endangered species, only the data on early juvenile growth have been reported (Guerao and Rotllant, 2010). However, no data are available from this early juvenile stage to sexual maturity for neither wild nor cultured individuals, and the only additional information available involves general biological traits and is based on data from commercial landings (Stevcic, 1963, Stevcic, 1968, Stevcic, 1971, Stevcic, 1973, Stevcic, 1976).

This paper reports the first successful occurrence of mating in the laboratory involving laboratory-born and laboratory-reared specimens of M. squinado. Egg masses and viable zoeae were obtained from these matings. This evidence demonstrates that the entire life cycle of the Mediterranean spider crab was completed in captivity. We report the first successful attempt to grow M. squinado from larvae to sexual maturity, and present the first growth data on individually monitored M. squinado to the age of sexual maturity. In addition, we have developed a growth model that incorporates the molting probability as a function of the temperature and the pre-molt size. This model has been coupled with a size-dependent model for predicting the molting increment and sexual maturity. Despite the use of individuals raised in aquaculture to parameterize the model, the data and model presented in this paper will be useful for managing the ongoing stocking program for M. squinado in the Balearic Islands (Durán et al., 2012), thus opening new opportunities for aquaculture to shift from an exclusive role in biomass production to an alternative approach to the sustainable management of stocks.

Section snippets

Materials and methods

Viable zoeae were obtained in the laboratory (Laboratori d'Investigacions Marines i Aqüicultura, LIMIA, Port d'Andratx, Mallorca) from wild female crabs in 2008, 2009 and 2010. First-instar juvenile crabs (C1) were reared together in mass culture tanks for 150–210 days. Subsequently, they were individually monitored until they were nearly 500 days old.

Results

The number of molts monitored per crab ranged from one to nine molts for both sexes. After a few outliers were removed from the data, 102 observations of MI and 75 observations of IP from 30 females (22 with more than one controlled molt) and 81 observations of MI and 62 observations of IP from 25 males (17 with more than one controlled molt) were considered for further analysis. The raw data (length and age of molts) are provided as Supplementary material.

Discussion

This paper reports the first successful occurrence of mating in the laboratory involving laboratory-born and laboratory-reared specimens of M. squinado. Egg masses and viable zoeae were obtained from these matings. This evidence demonstrates that the entire life cycle of the Mediterranean spider crab was completed in captivity. In addition, we present the first growth data on individually monitored M. squinado to the age of sexual maturity. We reported a long time series of high-quality

Acknowledgments

Financial support was provided by JACUMAR (“Cultivo de la centolla” and “Propuestas y mejoras para el diseño de los planes de repoblación y evaluación del impacto de los escapes” projects) and by the Government of the Balearic Islands. We are especially grateful to Dr. Amalia Grau for her help in histological observations and to the laboratory technicians, scholars and trainees at LIMIA for their help and support on the development of this study. Particular thanks go to three anonymous

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