Reduction of Δ1-pyrroline-2-carboxylic acid to proline by an escherichia coli proline auxotroph.
Barium hydroxide catalysed hydrolysis of 3 is shown to give 2 rather than 1 contrary to previous reports. The reduction of 2 to proline by an Escherichia coli proline auxotroph is shown by labelling experiments to proceed directly and not via initial isomerisation to 1, the natural precursor to proline in this organism.
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Design, synthesis, and biological activity of novel tetrahydropyrazolopyridone derivatives as FXa inhibitors with potent anticoagulant activity
2017, Bioorganic and Medicinal ChemistryCitation Excerpt :Subsequently, target compound 22c was afforded from 22b via hydrolysis, and the benzotriazole compound 23 was obtained via substitution of 18 with benzotriazole directly.25 The synthesis of target compounds 28a–28e is summarized in Scheme 5.26 Compound 12 was treated with trifluoroacetic anhydride in TEA to afford the nitrile compound 27, which was then subjected to reaction with different diamines to obtain the target compounds 28a–28e, respectively.
Directed evolution of novel biosynthetic pathways: Overexpression of leucine aminopeptidase allows an Escherichia coli proline auxotroph to grow faster on prolinamide
1994, Bioorganic and Medicinal Chemistry Letters