Reduction of Δ1-pyrroline-2-carboxylic acid to proline by an escherichia coli proline auxotroph.

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Abstract

A new synthesis of Δ1-pyrroline-2-carboxylic acid 2 and various isotopomers is described; the conversion of 2 to proline by Escherichia coli is shown to proceed by a direct reduction.

Barium hydroxide catalysed hydrolysis of 3 is shown to give 2 rather than 1 contrary to previous reports. The reduction of 2 to proline by an Escherichia coli proline auxotroph is shown by labelling experiments to proceed directly and not via initial isomerisation to 1, the natural precursor to proline in this organism.

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