Elsevier

Genomics

Volume 14, Issue 2, October 1992, Pages 398-402
Genomics

Detection of a polymorphism within the pepsinogen C gene with PCR: Construction of a linkage map around PGC from 6p11–6p21.3

https://doi.org/10.1016/S0888-7543(05)80232-0Get rights and content

An insertion/deletion polymorphism between exons 7 and 8 of the pepsinogen C gene (PGC), previously detectable with Southern analysis, was formatted for detection with PCR. Alleles were rapidly typed by UV irradiation of ethidium bromide-stained agarose gels. Whereas Southern analysis revealed two alleles, the smaller fragments generated with PCR allowed the resolution of three alleles that were previous, scored as a single allele and increased the heterozygosity of the system from 0.20 to 0.53. After a set of reference families was genotyped with the PCR-based polymorphism, a linkage map around the PGC gene on chromosome 6 was constructed. This included the HLA cluster and the highly informative D6S223 locus. PGC lies 22 cM proximal to HLA-DPB and between D6S5 and D6S4 at distances of 4.5 and 13.1 cM, respectively.

References (25)

  • ConneallyP.M. et al.

    Report of the committee on methods of linkage analysis and reporting

    Cytogenet. Cell Genet.

    (1985)
  • Donis-KellerH. et al.

    A genetic linkage map of the humana genome

    Cell

    (1987)
  • View full text