Characterization of hypothalamic low-voltage-activated Ca channels based on their functional expression in Xenopus oocytes
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2010, European Journal of PharmacologySelectivity signatures of three isoforms of recombinant T-type Ca<sup>2+</sup> channels
2007, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :Oocytes were used for the experiments 5 days after cRNA injection, since this time was required for maximal T-type Ca2+-channel current expression (data not shown). Membrane currents in the oocytes were recorded using a conventional double-microelectrode voltage-clamp technique as described before [17]. “Voltage recording” and “current passing” microelectrodes were pulled from borosilicate glass and had resistance of ∼ 3 MΩ and ∼ 1 MΩ, respectively, when filled with 3 M KCl.
Contrasting the effects of nifedipine on subtypes of endogenous and recombinant T-type Ca<sup>2+</sup> channels
2005, Biochemical PharmacologyCitation Excerpt :Stage V and VI oocytes from adult female Xenopus laevis frogs were used for the recombinant T-type Ca2+-channel expression. The procedures for oocyte isolation, maintenance and injection did not differ from those detailed elsewhere [10]. The volume of injected Cav3.1, Cav3.2 or Cav3.3 cRNA solution (0.2 μg/μl) was usually 50 nl per oocyte.