Immunohistochemical profiles of spinal lamina I neurones retrogradely labelled from the nucleus tractus solitarii in rat suggest excitatory projections
Section snippets
Surgical procedures
All procedures involving animal handling and usage were performed in accordance with the UK Animals (Scientific Procedures) Act 1986, European Council Directive 86/609/EEC, and the US Society for Neuroscience and NIH guidelines. Every effort was made to minimise any discomfort and suffering, and the minimum possible number of rats (n=11) was used to obtain reproducible and statistically significant results. Male Wistar rats (280–310 g; purpose bred at the University of Leeds) were anaesthetised
Injection sites
In eight animals used for the analysis, examination of sections through the medulla showed that the dense central cores of CTb immunoreactivity corresponding to the two injection sites lay within the commissural and medial subnuclei of the left NTS. The diffuse halos of immunoreactivity surrounding the points of injection filled the entire NTS, except for the most lateral parts at levels of the area postrema and more caudally, and extended to the ipsilateral dorsal motor nucleus of the vagus
Discussion
This study set out to define some of the putative neurotransmitters and other neuronal marker substances present in the different morphological categories of lamina I neurones that project to the NTS. GABA, glycine and glutamate were investigated, since these amino acid transmitters have previously been localised to neurones with a fusiform appearance,47., 63., 75., 81. and previous work has shown that this type accounts for the greatest number of marginal neurones projecting to the NTS in the
Acknowledgements
This work was supported by a British Heart Foundation project grant, a Medical Research Council JREI Award and a British Council “Treaty of Windsor” Travel Award. We are grateful to Dr Michael Conlon (Creighton University, Omaha, NE, USA), Dr Piers Emson (Babraham Institute, University of Cambridge, UK) and Dr David Pow (VTHRC, University of Queensland, Brisbane, Australia) for generously providing antisera used in this study.
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