Long-term changes in the distribution of galanin in dorsal root ganglia after sciatic or spinal nerve transection in rats
Section snippets
Experimental procedures
Female Wistar rats (Biological Resources Centre, UNSW; six to eight weeks of age at operation; 124–177 g) were used in this study. Under anaesthesia with i.p. ketamine 60 mg/kg plus xylazine 10 mg/kg, either the left sciatic nerve, or the ventral ramus of the left L5 spinal nerve, was ligated and cut peripherally. In four animals, Fast Blue (FB) was applied to the cut axons at the time of the sciatic lesion. Two (n=2), six (n=1), eight (n=3), 9–10 (n=6), 12 (n=2), 30 (n=2), 49 (n=1) and 55 (n=1)
Results
There were no detectable differences between naive control tissue and that from the side contralateral to the injury and both will be referred to as control.
Discussion
The present results confirm that GAL is upregulated in DRG somata after axotomy7 but also provide information about the long-term changes in GAL expression after both sciatic and spinal nerve lesions. This is the first report that GAL+ axons sprout within the DRGs containing neurones that project into damaged peripheral nerves. Previously, sprouting noradrenergic4., 20., 24. and SP/CGRP-containing19 axons and perineuronal rings have been described following various forms of nerve injury. The
Acknowledgements
This work was supported by grants from the National Health & Medical Research Council of Australia and by the Motor Accidents Authority of New South Wales. Drs Janet Keast and James Brock provided helpful comments on an earlier version of the manuscript.
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