Isolation of anti-mesothelin antibodies from a phage display library
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2012, Molecular ImmunologyCitation Excerpt :Following washing, the phage was eluted using an aqueous solution of 100 mM triethylamine and treated as described above for immobilized panning. To screen for specificity, recombinant antibody phage particles from single bacterial colonies were rescued in 96-well formats as described before (Chowdhury et al., 1997). Bacterial cultures were cooled to 4 °C, and the cells were removed by centrifugation at 5000 × g for 15 min at 4 °C.
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2007, Research in Veterinary ScienceA model system for optimising the selection of membrane antigen-specific human antibodies on intact cells using phage antibody display technology
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2001, Journal of Immunological MethodsCitation Excerpt :Immunolon 4 ELISA plates were coated overnight at 4°C with 100 μl of a 2 μg/ml solution of recombinant mesothelin (Chowdhury et al., 1997) or with BSA in 50 mM sodium bicarbonate buffer, pH 9.4–9.6.
Analysis of cloned Fvs from a phage display library indicates that DNA immunization can mimic antibody response generated by cell immunizations
1999, Journal of Immunological MethodsAn scFv phage clone that binds to human β<inf>2</inf>-microglobulin
1999, Immunotechnology
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