Elsevier

Methods in Enzymology

Volume 268, 1996, Pages 247-259
Methods in Enzymology

Stirred reactor with continuous nitric oxide sampling for use in kinetic studies

https://doi.org/10.1016/S0076-6879(96)68027-6Get rights and content

Abstract

We have described a novel apparatus for studying the rates of certain reactions of NO in aqueous solutions. A principal feature of this system is the ability to continuously measure NO concentrations, by employing a membrane inlet in conjunction with a chemiluminescence detector. The design variables that influence the performance of such systems have been discussed in some detail. This apparatus has already proved useful for determining the rate constants of several reactions in oxygenated NO solutions, and it is also well suited for quantitative studies using suspension cell cultures.

References (17)

  • R.S. Lewis et al.

    J. Biol. Chem.

    (1995)
  • A. Schumpe

    Chem. Eng. Sci.

    (1993)
  • D.L. Wise et al.

    Chem. Eng. Sci.

    (1968)
  • S.S. Mirvish

    Toxicol. Appl. Pharmacol.

    (1975)
  • S. Moncada et al.

    Pharmacol. Rev.

    (1991)
  • R.S. Lewis et al.

    Chem. Res. Toxicol.

    (1994)
  • R.S. Lewis et al.

    J. Am. Chem. Soc.

    (1995)
  • R.S. Lewis et al.

    Biol. Mass Spectrom.

    (1992)
There are more references available in the full text version of this article.

Cited by (0)

View full text