Extension of the electrostatic retention model of reversed-phase ion-pair chromatography to include the simultaneous effect of the organic modifier and the pairing ion
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Cited by (49)
Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
2022, Journal of Chromatography ACitation Excerpt :The retention mechanism in IP RP LC is complex issue, which can be described by electrostatic theory. Although the electrostatic theory has not been extended specifically to oligonucleotides yet, the retention principles of IP RP LC also apply to them [22–26]. The retention mechanism is based on interactions between positively charged IP agent (i.e. alkylamines) and negatively charged (thio)phosphate group of ONs [27].
Effect of ion-pairing reagent hydrophobicity on liquid chromatography and mass spectrometry analysis of oligonucleotides
2022, Journal of Chromatography ACitation Excerpt :The mechanism of IP RP LC was studied since 1970s for separations of charged small molecules [50,60–63]. Several hypotheses were proposed [23,50,51,60], where most assume an adsorption of hydrophobic IP reagent on the RP stationary phase, formation of a charged layer on the hydrophobic sorbent surface, and electrostatic retention of the charged analytes in addition to a hydrophobic retention mechanism. In this sense the IP RP LC resembles ion-exchange (IE) RP LC mixed-mode mechanism, where ionic and hydrophobic interactions contribute to retention simultaneously [64–66].
Sulfonium and phosphonium, new ion-pairing agents with unique selectivity towards polarizable anions
2006, Journal of Chromatography AIon-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: Retention prediction
2002, Journal of Chromatography A
- 1
On leave from the University of Chemical Engineering, Veszprem, Hungary.
- 2
Affiliated to the Institute of Physical Chemistry, University of Uppsala, Uppsala, Sweden.