NoteThe synthesis of 16-mercaptohexadecanyl glycosides for biosensor applications
Section snippets
General methods
Optical rotations were measured on a Perkin-Elmer 241 polarimeter in a 1 dm cell at ambient temperature (22±2 °C). Analytical thin-layer chromatography (TLC) was performed on Silica Gel 60-F254 (Merck) with detection by quenching of fluorescence and/or by charring with 10% H2SO4 in ethanol solution followed by heating at 180 °C. Millex-HV (0.45 μM) filter units were from Millipore (Missisuaga, ON). Column chromatography was performed on Silica Gel 60 (Merck, 40–60 μm), and solvents were distilled
Acknowledgements
This work was supported by the Protein Engineering (PENCE) and Canadian Bacterial Diseases (CBDN) Network Centers of Excellence. The authors thank Dr Elena Kitova for assistance in the analysis and purification of thiol and disulfide products.
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