RC+ gene-dependent inhibition of RNA synthesis without ppGpp accumulation

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Summary

The lack of a required amino acid or a block in the synthesis of an aminoacyl-tRNA inhibits net RNA synthesis and results in the accumulation of the nucleotides ppGpp and MSII in RC+ but not in RC cells of E.coli. It would be conceivable that ppGpp is the mediator of the inhibition of net RNA synthesis by the RC+ control system. However, the following results indicate that this is not (or at least not always) the case: Trimethoprim, an inhibitor of the formation of the peptide chain initiator formylmethionyl-tRNAf, blocks net RNA synthesis in RC+ but not in RC cells. We found that in RC+E.coli cells, trimethoprim (i) does not cause ppGpp or MSII accumulation if no required amino acid is missing, and (ii) does not inhibit ppGpp accumulation elicited by the lack of a required amino acid.

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This study has been supported by grants from the National Institutes of Health, and the American Cancer Society.

1

John H. Chen is at present in Ophthalmology Research, Columbia University, College of Physicians and Surgeons, New York, N.Y. 10032.

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