Gamete and embryo isolation and culture with microfluidics
Section snippets
Microfluidics
Microfluidics is a relatively new technology that shows promise as an alternative in the process of embryo in vitro production (IVP) [1], [2], [3], [4], [5], [6], [7], [8]. Microfluidics is defined as the study of physical principles of fluid behavior in a microenvironment and has been used extensively in chemistry [9] and molecular biology [10]. At least two potential advantages can be realized by working at the micro-level. First, mechanical advantages can be achieved at the micro-scale that
Development of a dynamic culture system for mammalian embryos
The mammalian preimplantation embryo resides within an in vivo developmental environment markedly different from soluble and non-soluble environments provided in standard culture. In vivo the developmental environment is microscopic, moist yet not fully-fluid, mechanically dynamic, with surface structures that are moving and glycoprotein rich [24]. In standard mammalian embryo culture procedures, embryos are manipulated in a macroscopic fully-fluid environment that is mechanically stagnant,
Microfluidics and IVP of the future
Although most works using microfluidics in IVF have been performed in a step-wise fashion, the ultimate goal of process miniaturization and microfluidic technology is integration. Use of microfluidic technology for sperm processing ultimately results in a small volume and fraction of motile sperm. Such volumes are difficult to subsequently utilize and translate into a macroscale environment. However, laminar flow-sorted sperm have been used for subsequent fertilization and intracytoplasmic
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Cited by (39)
A tilting embryo culture system increases the number of high-grade human blastocysts with high implantation competence
2013, Reproductive BioMedicine OnlineCitation Excerpt :The development of a reliable culture system to increase the number of usable embryos with high implantation competence in one oocyte retrieval cycle is critically important for IVF and embryo transfer. Some reports of culture systems to control chemical and mechanical microenvironments for in-vitro mammalian embryo culture have been published, such as a microwell approach (Vajta et al., 2000; Hashimoto et al., 2009; Ebner et al., 2010), pulsative mechanical microvibration (Isachenko et al., 2010; Mizobe et al., 2010) and dynamic culture systems with fluid motion (Suh et al., 2003; Cabrera et al., 2006; Smith and Takayama, 2007; Blockeel et al., 2009; Heo et al., 2010; Smith et al., 2011; Swain and Smith, 2011). For human embryo culture, microwell culture increases the cell number of the inner cell mass in blastocysts (Hashimoto et al., 2009), and pulsative mechanical microvibration improves the pregnancy rate regardless of the day of embryo transfer (Isachenko et al., 2010).
Microfluidic mixing for sperm activation and motility analysis of pearl Danio zebrafish
2012, TheriogenologyCitation Excerpt :Microfluidic platforms with microfabrication technologies have been used to shorten analysis times, reduce volumes of reagents, and enable new discoveries in cell biology [15–17]. These devices have been utilized in studies of gamete and embryo physiology where microchannels have been used for reliable delivery of sperm to oocytes [18], gamete and embryo isolation and culture [19,20], sperm sorting and separation [21–27], and manipulation of oocytes and sperm [28–30]. However, to date there have been no reports regarding the use of microfluidics to activate and analyze aquatic species sperm cells.
Lab-on-a-chip spectrophotometric characterization of porcine oocytes
2012, Sensors and Actuators, B: Chemical