Molecular Cell
Volume 70, Issue 6, 21 June 2018, Pages 1008-1024.e6
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Article
UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F

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Highlights

  • Neddylation inhibitor MLN4924 increases UBE2M levels but decreases UBE2F levels

  • UBE2M is stress inducible by hypoxia and mitogen stimulation via HIF-1α and AP1

  • UBE2M acts as a neddylation E2 to activate CUL3/Keap1 E3 for UBE2F degradation

  • UBE2M acts as a ubiquitin E2 for Parkin/DJ-1 E3 to degrade UBE2F upon stress stimuli

Summary

UBE2M and UBE2F are two family members of neddylation E2 conjugating enzyme that, together with E3s, activate CRLs (Cullin-RING Ligases) by catalyzing cullin neddylation. However, whether and how two E2s cross-talk with each other are largely unknown. Here, we report that UBE2M is a stress-inducible gene subjected to cis-transactivation by HIF-1 and AP1, and MLN4924, a small molecule inhibitor of E1 NEDD8-activating enzyme (NAE), upregulates UBE2M via blocking degradation of HIF-1α and c-JUN. UBE2M is a dual E2 for targeted ubiquitylation and degradation of UBE2F, acting as a neddylation E2 to activate CUL3-Keap1 E3 under physiological conditions but as a ubiquitylation E2 for Parkin-DJ-1 E3 under stressed conditions. UBE2M-induced UBE2F degradation leads to CRL5 inactivation and subsequent NOXA accumulation to suppress the growth of lung cancer cells. Collectively, our study establishes a negative regulatory axis between two neddylation E2s with UBE2M ubiquitylating UBE2F, and two CRLs with CRL3 inactivating CRL5.

Keywords

cullin 3
DJ-1
Keap1
neddylation
Parkin
UBE2F/UBE2M
stress responsiveness
and ubiquitylation

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These authors contributed equally

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