Regular paper
cAMP-activated chloride channels in a MR-transfected pancreatic adenocarcinoma-derived cell line, pANS6

https://doi.org/10.1016/0925-4439(95)00047-8Get rights and content
Under an Elsevier user license
open archive

Abstract

Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.

Keywords

CFTR, cystic fibrosis transmembrane regulator
cAMP activation
Chloride channel
Adenocarcinoma, pancreatic

Cited by (0)