Expression of nerve growth factor receptor mRNA is developmentally regulated and increased after axotomy in rat spinal cord motoneurons

doi:10.1016/0896-6273(89)90049-4

Neuron

Volume 2, Issue 6, June 1989, Pages 1605-1613

https://doi.org/10.1016/0896-6273(89)90049-4 Get rights and content

Abstract

In situ hybridization histochemistry and RNA blot analysis were used to study expression of nerve growth factor receptor (NGF-R) mRNA in rat spinal cord motoneurons. The results show that NGFR mRNA is expressed at high levels in rat spinal cord motoneurons at the time of naturally occurring cell death. This expression is sustained, but reduced, during synapse formation and is subsequently greatly reduced in the adult spinal cord. A unilateral crush lesion of the sciatic nerve resulted in an 8-fold increase in NGF-R mRNA in adult rat spinal cord motoneurons 3 days after lesion, compared with the nonlesioned side. NGF-R mRNA induction was even more pronounced 7 and 14 days after lesion, reaching levels 12 times higher than those on the nonlesioned side. However, 6 weeks after lesion, when the motor function of the leg was largely restored, NGF-R expression had decreased to levels similar to those on the contralateral side. We therefore suggest that NGFR mediates a trophic or axonal guidance function for developing and regenerating spinal cord motoneurons.

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Neuron

PC12 cellExpression of nerve growth factor receptor mRNA is developmentally regulated and increased after axotomy in rat spinal cord motoneurons

Abstract

Brain Res.

J. Biol. Chem.

Dev. Biol.

Dev. Biol.

Neuron

J. Biol. Chem.

Neurosci. Lett.

Brain Res.

Brain Res.

Cell

Dev. Brain Res.

Brain Res.

Trends Neurosci.

Brain Res.

J. Biol. Chem.

Brain Res.

Brain Res.

Brain Res.

Dev. Biol.

Binding of nerve growth factor in superior cervical ganglia

Purification of a new neurotrophic factor from mammalian brain

EMBO J.

Purification of the chick eye ciliary neuronotrophic factor

J. Neurochem.

Cell death in neuronal development: regulation by trophic factors

Retrograde transport of nerve growth factor in chicken embryo

Developmentally regulated expression of the nerve growth factor receptor in the periphery and brain

Muscle-derived factors that support survival and promote fiber outgrowth from embryonic chick spinal motor neurons in culture

Dev. Biol.

Retrograde axonal transport of specific macromolecules as a tool for characterizing nerve terminal membranes

J. Neurobiol.

Detection of nerve growth factor mRNA during chicken embryo development

Development

Differentiation and degeneration in the motor horn of the foetal mouse

J. Morphol.

Cell death in development of the lateral motor column of the chick embryo

J. Comp. Neurol.

Naturally occurring neuronal death in vertebrates

Neurosci. Commun.

Properties of the β-nerve growth factor receptor of avian dorsal root ganglia

Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection

J. Cell Biol.

PC12 cell
Expression of nerve growth factor receptor mRNA is developmentally regulated and increased after axotomy in rat spinal cord motoneurons