Three brain sodium channel α-subunit genes are clustered on the proximal segment of mouse chromosome 2

doi:10.1016/0888-7543(91)90450-S

Genomics

Volume 10, Issue 3, July 1991, Pages 666-672

https://doi.org/10.1016/0888-7543(91)90450-S Get rights and content

Abstract

We have used long-range physical mapping and restriction fragment length polymorphisms between two mouse species to determine the chromosomal organization and location of the genes encoding three distinct isoforms of the α-subunit of the brain sodium channel. Physical mapping by pulsed-field gel electrophoresis has established that Scn2a and Scn3a (genes encoding type II and type III sodium channel α-subunit isoforms) are physically linked and are separated by a maximum distance of 600 kb. The segregation of restriction fragment length variations in backcross progeny of a Mus musculus and Mus spretus mating indicates that Scn1a (gene encoding the type I sodium channel α subunit) and Scn2a are tightly linked and are separated by a distance of 0.7 cM. Linkage analysis in backcross and recombinant inbred (BXD and AKXD) strains of mice localized the three sodium channel genes to the proximal segment of mouse chromosome 2 and suggested the probable gene order centromere-Hc-Neb-Pmv7-Scn2a/Scn3a-Scn1a-Mpmv14. These results indicate that the three isoforms of the brain sodium channel α-subunit are encoded by three distinct genes that share a common ancestral origin.

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View full text

Three brain sodium channel α-subunit genes are clustered on the proximal segment of mouse chromosome 2

Abstract

Anal. Biochem

Neuron

FEBS Lett

Genomics

Neuron

Genomics

Assignment of the human acetylcholine receptor β subunit gene to chromosome 17 and the α and δ subunit to chromosome 2

Cytogenet. Cell Genet

Human homeo box-containing genes located at chromosome regions 2q31 → 2q37 and 12q12 → 12q13

Am. J. Hum. Genet

Structure and function of voltage-sensitive ion channels

Science

Separation of large DNA molecules by contour-clamped homogeneous electric fields

Science

Modulation of sodium channel mRNA levels in rat skeletal muscle

Chromosomal location of the genes encoding complement components C5 and factor H in the mouse

J. Immunol

The structural and functional organization of the murine HOX gene family resembles that of Drosophila homeotic genes

EMBO J

The gene for the muscle nicotinic acetylcholine receptor alpha subunit maps to chromosome 2, not chromosome 17

Mouse News Lett

Hox-5.1 defines a homeobox-containing gene locus on mouse chromosome 2

Hyperkalemic periodic paralysis and the adult muscle sodium channel α-subunit gene

Science