Elsevier

Gene

Volume 161, Issue 2, 19 August 1995, Pages 301-302
Gene

Brief note
Isolation of a cDNA encoding a mouse TFIID subunit containing histone H4 homology

https://doi.org/10.1016/0378-1119(95)00285-EGet rights and content

Abstract

A cDNA encoding a mouse transcription factor IID (TFIID) subunit, containing histone H4 homology, was cloned and sequenced. The predicted 678-amino-acid (aa) sequence of this molecule showed 97 and 41% identity to the human and Drosophila melanogaster homologues, respectively. Four putative direct repeats were found in the most highly conserved region in the central part of this protein.

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  • Expression of TAFII70 RNA and protein during oogenesis and development of the amphibian Pleurodeles waltl

    2000, Mechanisms of Development
    Citation Excerpt :

    The identified clone (PwTAFII70) is 2790 bp long and contains an ORF encoding a 649 amino acid protein with an estimated molecular weight of 68.3 kDa. Fig. 1 shows the degree of identity of predicted protein with TAFII60/70 isolated from other organisms (Weinzierl et al., 1993; Kokubo et al., 1994; Hasegawa et al., 1995; Hisatake et al., 1995; Kida et al., 1995; Poon et al., 1995; Umehara et al., 1995). PwTAFII70 mRNA, which is expressed as a 3 kb transcript throughout development, is abundant in mature oocytes and during cleavage, but has lower levels of expression in late embryos (Fig. 2).

On request, the authors will provide detailed experimental evidence for the conclusions reached in this Brief Note.

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