Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA

doi:10.1016/0378-1119(90)90020-R

Gene

Volume 89, Issue 2, 14 May 1990, Pages 289-294

https://doi.org/10.1016/0378-1119(90)90020-R Get rights and content

Abstract

Vertebrate sarcomeric myosin heavy chains (MHC) are encoded by multigene families whose members show tissue-specific and developmentally-regulated patterns of expression. Molecular genetic studies have allowed the cloning of a small number of complete genes or cDNAs encoding MHC isoforms [see Warrick and Spudich, Annu. Rev. Cell Biol. 3 (1987) 379-421]. Reported here is the isolation and sequence of a 2.6-kb cDNA that encodes the subfragment 1 or head of a human perinatal skeletal MHC. A cDNA sequence encoding the rod portion of this isoform has been previously reported [Feghali and Leinwand, J. Cell Biol. 108 (1989) 1791-1797]. Polymerase chain reaction with fetal skeletal muscle RNA was used to join the two nonoverlapping cDNA sequences to construct a full-length sequence. The gene encoding the perinatal skeletal MHC has been localized to the cluster of skeletal MHC-encoding genes on chromosome 17.

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Short communicationGeneration of a full-length human perinatal myosin heavy-chain-encoding cDNA

Abstract

J. Mol. Biol.

J. Biol. Chem.

J. Biol. Chem.

Biochim. Biophys. Acta

J. Mol. Biol.

Cell

Developmental appearance of myosin heavy chain and light chain isoform in vivo and in vitro in chicken skeletal muscles

Dev. Biol.

Nucleotide sequence of full length human embryonic myosin heavy chain cDNA

Nucleic Acids Res.

Molecular genetic characterization of a developmentally regulated human perinatal myosin heavy chain

J. Cell Biol.

Ribonucleic acid isolated by cesium chloride centrifugation

Biochemistry

Expression and DNA sequence analysis of a human embryonic skeletal muscle myosin heavy chain gene

Nucleic Acids Res.

Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase

Biotech.

Complete nucleotide sequence of full length cDNA for rat beta myosin heavy chain

Nucleic Acids Res.

Isolation and characterization of human myosin heavy chain genes

Short communication
Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA