Time course of neurofilament protein loss following depolarization-induced injury in CNS culture

doi:10.1016/0304-3940(95)11921-I

Neuroscience Letters

Volume 197, Issue 2, 8 September 1995, Pages 159-163

https://doi.org/10.1016/0304-3940(95)11921-I Get rights and content

Abstract

In septo-hippocampal cell cultures, brief potassium depolarization produces calcium-dependent decreases in neurofilament proteins and loss of fine neuritic processes within 24 h. It is not known whether neurons later exhibit delayed degeneration and die, live with enduring neurofilament loss, or restore neurofilament protein levels. Therefore, we exposed septohippocampal cultures to 6 min potassium depolarization (60 mM) with 2.8–11.8 mM extracellular Ca²⁺ and evaluated immunoreactivity for low, medium and heavy neurofilament proteins, neuronal number, and neuronal morphology for 10 days. Neuronal number remained unchanged; neurofilament protein levels recovered to between 31% and 99% of control levels, and fine neuritic processes reappeared.

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Time course of neurofilament protein loss following depolarization-induced injury in CNS culture

Abstract

Brain Res.

Neuroscience

Neurosci. Lett.

Trends Neurosci.

Brain Res.

Neuroscience

Cell Biol.

Brain Res.

Brain Res.

Brain Res.

Trends Neurosci.

Glutamate neurotoxicity in cortical cell culture

J. Neurosci.

Phosphorylated neurofilament antigens in neurofibrillary tangles in Alzheimer's disease

J. Neuropathol. Exp. Neurol.

Ionic changes and alterations in the size of the extracellular space during epileptic activity

Adv. Neurol.

An increase in phosphorylation of microtubule associated protein 2 accompanies dendrite extension during the differentiation of cultured hippocampal neurones

Eur. J. Biochem.

Requirement for BDNF in activity-dependent survival of cortical neurons

Science

Combined oxygen and glucose deprivation in cortical cell culture; calcium-dependent and calcium-independent mechanisms of neuronal injury

J. Neurosci.

Neuron-glia relationships in human and experimental epilepsy: a biochemical point of view

Adv. Neurol.

Distribution of calpains 1 and 2 in rat brain

J. Neurosci.

Alterations of a 200 kDa neurofilament in the rat hippocampus after forebrain ischemia

J. Cerebral Blood Flow Metab.