Elsevier

Neuroscience Letters

Volume 168, Issues 1–2, 28 February 1994, Pages 23-28
Neuroscience Letters

Single-channel activity correlated with medium-duration, Ca-dependent K current in cultured rat hippocampal neurones

https://doi.org/10.1016/0304-3940(94)90407-3Get rights and content

Abstract

Whole-cell voltage- and patch-clamp techniques were used to record the calcium-dependent component of ImAHP and correlated single-channel activity in postnatal cultured rat hippocampal neurons. The Ca-dependent ImAHP was elicited by voltage steps to + 10 mV from a holding potential of −50 mV in the whole-cell mode. In cell-attached patches, single currents of ∼2 pA were observed following spontaneous action currents. The duration of ensemble-averaged single-channel activity was very similar to that of the whole-cell ImAHP, ∼100 ms. Both whole-cell current and single-channel activity could be blocked by bath-applied cadmium, 200 μM. Vigorous channel activity was evoked by bursts of action potentials that are known to elicit Ca-dependent AHPs. We conclude that this channel is a candidate for mediating ImAHP.

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