Elsevier

Neuroscience Letters

Volume 155, Issue 2, 11 June 1993, Pages 136-139
Neuroscience Letters

Molecular cloning of a human neuronal nicotinic acetylcholine receptor β3-like subunit

https://doi.org/10.1016/0304-3940(93)90691-DGet rights and content

Abstract

Three cDNA clones homologous to rat neuronal nicotinic receptors were identified in a human brain stem library by screening at low stringency with a mixture of rat α5, β2 and β4 subunit probes. Positive clones were further analysed by hybridisation at higher stringency with individual rat nicotinic receptor probes. One positive clone was found to encode a protein that exhibited characteristic features of a member of the ligand-gated ion channel class of protein subunits, and showed 89% homology with a rat β3 neuronal nicotinic receptor subunit. Northern blots demonstrated the presence of a 1.7 kb transcript in RNA extracted from adult human Pons. This clone has therefore been designated the human β3 neuronal nicotinic receptor subunit.

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