Elsevier

Molecular Brain Research

Volume 28, Issue 1, January 1995, Pages 94-100
Molecular Brain Research

Research report
Antibodies to the rat β3 subunit of the γ-aminobutyric acidA receptors

https://doi.org/10.1016/0169-328X(94)00188-KGet rights and content

Abstract

Polymerase chain reaction was used to amplify the cDNA region that codes for the large intracellular loop of the β3 subunit of the γ-aminobutyric acidA/benzodiazepine receptors (GABAAR/BZDR) from rat brain. The amplified cDNA was inserted into the prokaryotic expression vector pGEX-3X and a fusion protein containing glut athione-S-transferase and β3 intracellular loop moieties was expressed in bacteria. The fusion protein was affinity-purified and it was used to raise a rabbit anti-β3 antiserum. The anti-β3 antiserum immunoprecipitated the γ-aminobutyric acidA receptor from rat and bovine brain. Immunoblots of the affinity-purified GABAAR/BZDR from bovine brain revealed that the anti-β3 antiserum reacted with a 57 kDa peptide, whereas the monoclonal antibody 62-3G1 that recognized both β2 and β3 reacted with 55 and 57 kDa peptides. The anti-β3 antiserum showed specificity for the β3 subunit vs β2 and β1.

References (33)

  • S. Endo et al.

    Preparation of antibodies to β subunits of the γ-aminobutyric acidA receptors

    J. Neurochem.

    (1992)
  • Fernando, L.P., Khan, Z.U., McKernan, R.M. and De Blas, A.L., Monoclonal antibodies to the human γ2 subunit of the...
  • Q. Gu et al.

    Immunocytochemical study of GABAA receptors in the cat visual cortex

    J. Comp. Neurol.

    (1993)
  • A. Gutiérrez et al.

    Immunocytochemical localization of the γ2 short and γ2 long subunits of the GABAA receptor in the rat brain

    J. Neurosci.

    (1995)
  • P. Häring et al.

    Monoclonal antibodies reveal structural homogeneity of γ-aminobutyric acid /benzodiazepine receptors in different brain area's

  • Z.U. Khan et al.

    Antibodies to the human γ2 subunit of the GABAA receptor

    J. Neurochem.

    (1993)
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