Postmortem stability of dopamine D1 receptor mRNA and D1 receptors

doi:10.1016/0169-328X(93)90092-4

Molecular Brain Research

Volume 18, Issue 4, June 1993, Pages 290-296

https://doi.org/10.1016/0169-328X(93)90092-4 Get rights and content

Abstract

The effects of postmortem interval on dopamine D₁ mRNA and D₁ receptors were assessed in rat striatum under conditions simulating the handling of human brain tissue at 0, 6, 12, and 24 h postmortem. The amount of D₁ mRNA was measured by both in situ hybridization film and emulsion autoradiography with [³⁵S]dATP-labeled oligonucleotide probes. D₁ receptor density was determined by autoradiography with [¹²⁵I]SCH 23982. Neither the total amount of D₁ mRNA in the striatum nor the frequency distribution of striatal cells expressing D₁ mRNA varied with the postmortem interval. There was a modest but significant decrease (ca. 10%) in D₁ receptors over the 24 h postmortem interval; this decrease occurred within the first 6 h postmortem, with no further decreases up to 24 h postmortem. These findings suggest that the effects of postmortem interval on D₁ mRNA and receptors are minimal and should not limit an examination of possible alterations in dopamine D₁ receptor mRNA and D₁ receptors in the postmortem brains of humans with neuropsychiatric disease.

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Research reportPostmortem stability of dopamine D1 receptor mRNA and D1 receptors

Abstract

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Eur. J. Pharmacol.

Pharmac. Ther.

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J. Receptor Res.

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Extensive postmortem stability of RNA from rat and human brain

J. Neurosci. Res.

Multiple receptors for dopamine

Nature

Dopamine receptor turnover rates in rat striatum are age dependent

Research report
Postmortem stability of dopamine D₁ receptor mRNA and D₁ receptors

Alternative splicing directs the expression of two D₂ dopamine receptor isoforms