A serially transplantable human giant cell glioblastoma that maintains a near-haploid stem line

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Abstract

We have karyotyped a human giant cell glioblastoma removed from an 11-year-old girl and have established from it a subcutaneously transplantable line in athymic nude mice. The original tumor contained near-haploid cells with 25 or 26 chromosomes, including two copies of #1, (7 or 7p+) and #18. There were also hyperdiploid (49–52) cells that were tetraploid for these same three chromosome types; doubled versions of the hyperdiploid population were also seen. The stemline of the mouse-grown tumor was 26,X,+1,+7p+,+18 in the first passage and has remained consistently near-haploid through ten serial in vivo passages. Growth stabilization has occurred with an average latency of less than 3 months. This transplantable line is available for evaluating chemotherapeutic responsiveness of human giant cell glioblastoma and for studying near-haploidy in solid human tumors.

References (28)

  • JW Kernohan et al.

    Sarcomas of the Brain

  • KJ Zulch et al.

    Pathology and classification of gliomas

    Prog Neurol Surg

    (1968)
  • PE Duffy et al.

    Glial fibrillary acidic protein in giant cell tumors of brain and other gliomas: A possible relationship to malignancy, differentiation and pleomorphism of glia

    Acta Neuropathol (Berl)

    (1980)
  • SC Schold et al.

    Treatment of five subcutaneous human glioma tumor lines in athymic mice with carmustine procarbazine and mithramycin

    Cancer Treat Rep

    (1983)
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