Intracellular plasma proteins in human fetal choroid plexus during development II. The distribution of prealbumin, albumin, alpha-fetoprotein, transferrin, IgG, IgA, IgM, and alpha1-antitrypsin

doi:10.1016/0165-3806(82)90025-6

Developmental Brain Research

Volume 3, Issue 2, February 1982, Pages 251-262

https://doi.org/10.1016/0165-3806(82)90025-6 Get rights and content

Abstract

Individual choroid plexuses of human fetal brain at different developmental stages were investigated by an indirect immunoperoxidase technique. The number and distribution of prealbumin-, albumin-, alpha-fetoprotein-, transferrin-, IgG-, IgA-, IgM- and alpha₁-antitrypsin-containing choroid plexus epithelial cells were recorded.

The distribution of prealbumin in choroid plexus epithelial cells differed from that of other plasma proteins identified in these cells. Prealbumin was present in more than 90% of the cells at all ages examined in contrast to albumin, alpha-fetoprotein, transferrin and IgG, which were found in less than 40% of the cells, and this proportion declined later in gestation to only a few per cent. The telencephalic plexus exhibited a staining pattern for the proteins, except for prealbumin, which was different from that of the diencephalic and myelencephalic plexuses. Double-staining and staining with polyvalent antiserum indicated that the same epithelial cells showed positive reaction for albumin, alpha-fetoprotein, transferrin and IgG.

The results are related to the observation of a high concentration of plasma protein in fetal CSF which may result from transcellular transfer from blood to CSF across the choroid plexus epithelial cells.

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Research reportIntracellular plasma proteins in human fetal choroid plexus during development II. The distribution of prealbumin, albumin, alpha-fetoprotein, transferrin, IgG, IgA, IgM, and alpha1-antitrypsin

Abstract

Develop. Biol.

Develop. Biol.

Comp. Biochem. Physiol.

Develop. Brain. Res.

Neurosci. Lett.

Neurosci. Lett.

Exp. Eye Res. (Lond.)

Trends Neurosci.

Levels of plasma proteins in human and rat fetal CSF and the development of the blood-CSF barrier

Neuropädiatrie

Fine structure observations of the uptake of intravenously injected peroxidase by the rat choroid plexus

J. Histochem. Cytochem.

Evidence for intracellular localization of alpha-fetoprotein in the developing rat brain

Brain Res.

Quantitation of plasma proteins in cerebrospinal fluid

Scand. J. Immunol.

Junction between intimately apposed cell membranes in the vertebrate brain

J. Cell. Biol.

Demonstration of hepatitis B-surface antigen in liver biopsies

Acta pathol. microbiol. scand.

Ultrastructural differentiation of the junctional complex of the avian choroidal epithelium

J. comp. Neurol.

Proteins in cerebrospinal fluid and plasma of fetal sheep during development

J. Physiol. (Lond.)

Plasma proteins in fetal sheep brain: blood-brain barrier and intracerebral distribution

J. Physiol. (Lond.)

Research report
Intracellular plasma proteins in human fetal choroid plexus during development II. The distribution of prealbumin, albumin, alpha-fetoprotein, transferrin, IgG, IgA, IgM, and alpha₁-antitrypsin