The use of ultra-violet setting glue for microelectrode fabrication

doi:10.1016/0165-0270(80)90026-6

Journal of Neuroscience Methods

Volume 3, Issue 2, December 1980, Pages 203-204

https://doi.org/10.1016/0165-0270(80)90026-6 Get rights and content

Abstract

The characteristics of glues which set in ultra-violet light are described with respect to their advantages for microelectrode assembly.

References (3)

A.R. Crossman et al.
Problems associated with iontophoresis studies in the caudate nucleus and substantia nigra
Neuropharmacology
(1974)

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A 60Co source with the photon energy of about 1.2 MeV was used [7–9]. For bonding onto the insulator substrate that is transparent for the bulk luminescence of Cd0.9Zn0.1Te crystals, a glue compound was used, fast solidifying under ultraviolet radiation [10]. In the as-grown CdZnTe material, there are many Cd vacancies and residual donors (atoms of III–VII group metals) [4,9].
A photoluminescence method was used for characterization of crystalline perfection of CdZnTe single crystals in the different stages of an up-to-date process of ionizing radiation sensor production. It was shown that the point method is an effective tool for diagnostics of technology-induced defects and structural irregularities, which can have substantial effect on the sensor performance. From the photoluminescence spectrum, one can obtain information about nature and relative concentrations of initial (as-grown) and technology-induced defects as well as about their interaction. Therefore, the photoluminescence method gives the possibility to correct technological regimes and thus support high quality of the material and high spectrometric (energy resolution, sensitivity) performance of sensor devices.
A simple and comprehensive method for the construction, repair and recycling of single and double tungsten microelectrodes
1995, Journal of Neuroscience Methods
A method for the construction, repair and recycling of tungsten-in-glass micro-electrodes is described. Essentially, the method employs insulating tungsten electrodes by collapsing borosilicate-glass capillaries onto tungsten wires. Procedures are described for either (1) removing excess glass insulation from the microelectrode tip or (2) fine adjustment and reshaping of the exposed electrode tip. This approach enables the controlled shaping of the electrode tip during preparation and/or reshaping the electrode tip or glass insulation of damaged electrodes. The method may be applied in the preparation of bipolar tungsten-in-glass electrode and tungsten electrode/micropipette assemblies. Advantages of the method are discussed.
A combined recording and microiontophoresis technique for input-output analysis of single neurons in the mammalian CNS
1988, Journal of Neuroscience Methods
A method is described for producing electrolytically sharpened and lacquer-insulated steel microelectrodes and for their attachment to a multibarrel micropipet assembly by light-curing bonding compound. This electrode complex is specially designed for recordings from deep brain structures. It permits the analysis of the input signals to and the output signals from a single neuron recorded simultaneously from an extracellular electrode position, termed juxtacellular recording. Furthermore it provides the ability to manipulate pharmacologically the input-output ratio expressed by the neuron under varying sequences of natural stimulation.
Zetidoline blocks dopamine autoreceptors in the rat substantia nigra
1985, Neuropharmacology
Zetidoline, applied microiontophoretically onto neurones in the substantia nigra, zona compacta of anaesthetized rats, reversibly blocked the depressant effect of iontophoretically-applied dopamine. The inhibitory actions of γ-aminobutyric acid (GABA) and glycine and the excitatory action of glutamate were unaffected by the antagonist.
Zetidoline applied by bath perfusion, was similarly an antagonist of the inhibitory effect of bath applications of dopamine on neurones in the zona compacta of slices of substantia nigra; the pA₂ for zetidoline in this preparation was 7.0.
The history and development of microiontophoresis in experimental neurobiology
1984, Progress in Neurobiology
The electrophysiology of dopamine (D<inf>2</inf>) receptors: A study of the actions of dopamine on corticostriatal transmission
1983, Neuroscience
Electrophysiological recordings from the cells of the neostriatum in rats anaesthetised with halothane revealed only inhibitory actions of dopamine applied iontophoretically close to the cells. Inhibition of cortical driving seemed to have a slightly higher threshold in most cells but dopamine inhibited spontaneous action potentials, glutamate-induced responses, and cortical driving in the cells studied. Fluphenazine applied iontophoretically blocked the actions of dopamine but was itself without effect on the neuronal responses. Sulpiride, in contrast, was without effect on the spontaneous activity of the cells and was ineffective in blocking the action of applied dopamine. Sulpiride, nevertheless, increased the response to cortical stimulation though it had no action on the response to applied glutamate.
These results suggest that the sub-class of dopamine receptors on the terminals of the corticostriatal pathway may be inhibitory on glutamate release and preferentially sensitive to blockade by sulpiride.

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Letter to the editorThe use of ultra-violet setting glue for microelectrode fabrication

Abstract

Neuropharmacology

Letter to the editor
The use of ultra-violet setting glue for microelectrode fabrication