The family of genes encoding odorant receptors in the channel catfish

doi:10.1016/0092-8674(93)90395-7

Cell

Volume 72, Issue 5, 12 March 1993, Pages 657-666

https://doi.org/10.1016/0092-8674(93)90395-7 Get rights and content

Abstract

The anatomical and numerical simplicity of the fish olfactory system has led us to examine the family of olfactory receptors expressed in the catfish. We have identified a family of genes encoding seven transmembrane domain receptors that share considerable homology with the odorant receptors of the rat. The size of the catfish receptor repertoire appears to be far smaller than in mammals. Analysis of the nucleotide sequences suggests that these receptor genes have undergone positive Darwinian selection to generate enhanced diversity within the putative odorant-binding domains. Individual receptor clones anneal with 0.5%–2% of the olfactory neurons, suggesting that a single cell expresses only a small subset of distinct odorant receptors. Each cell, therefore, possesses a unique identity defined by the receptors it expresses. These data suggest that the brain may discriminate among odors by determining which neurons have been activated.

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^★: Present address: Division of Neurobiology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720.

^†: Present address: Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115.

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Cell

ArticleThe family of genes encoding odorant receptors in the channel catfish

Abstract

Comp. Biochem. Physiol. B

Cell

Anal. Biochem.,

Biochim. Biophys. Acta

Neuron

Prog. Neurobiol.,

J. Steroid Biochem. Mol. Biol.,

FEBS Lett.,

Meth. Enzymol.,

Neuron

J. Biol. Chem.,

Cell

Cell

Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants

EMBO J.

Rapid kinetics of second messenger formation in olfactory transduction

Nature

Biochemical studies of olfaction: binding specificity of radioactively labeled stimuli to an isolated olfactory preparation from rainbow trout (Salmo gairdneri)

Electrophysiological evidence for acidic, basic, and neutral amino acid olfactory receptor sites in the catfish

J. Gen. Physiol.,

Electro-olfactogram and multiunit olfactory receptor responses to binary and trinary mixtures of amino acids in the channel catfish Ictalurus punctatus

J. Gen. Physiol.,

Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons

Nature

Ligand binding to the β-adrenergic receptor involves its rhodopsin-like core

Nature

Functional properties of the fish olfactory system

Prog. Sens. Physiol.

Article
The family of genes encoding odorant receptors in the channel catfish