Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly

doi:10.1016/0092-8674(93)90120-F

Cell

Volume 72, Issue 3, 12 February 1993, Pages 443-457

https://doi.org/10.1016/0092-8674(93)90120-F Get rights and content

Abstract

We have generated temperature-sensitive lethal point mutations in the small nucleolar RNA-associated protein fibrillarin (encoded by the NOP1 gene in yeast) and analyzed their effects on ribosome synthesis. The five alleles tested all prevent synthesis of normal ribosomes, but in dramatically different ways. At the non-permissive temperature, the nop1.2 and nop1.5 alleles prevent synthesis of both 18S and 25S rRNA and all pre-rRNA species except the 35S primary transcript. In contrast, the nop1.3, nop1.4, and nop1.7 alleles do not strongly impair processing. In nop1.3 strains, nucleolar methylation of pre-rRNA is strongly inhibited; late, cytoplasmic methylation of 18S rRNA and tRNA methylation continue. The nop1.4 and nop1.7 alleles result in the synthesis of cytoplasmic 60S ribosomal subunits with strongly aberrant mobilities on sucrose gradients even at the permissive temperature, owing to the impairment of a late step in ribosome assembly. Thus, all major posttranscriptional activities in ribosome synthesis, pre-rRNA processing, pre-rRNA modification, and ribosome assembly are dependent on fibrillarin.

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Cell

ArticleTemperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly

Abstract

Biochem. Biophys. Res. Commun.

J. Biol. Chem.

J. Biol. Chem.

Cell

FEBS Lett.

Biochim. Biophys. Acta

J. Biol. Chem.

J. Mol. Biol.

Prog. Nucl. Acids Res. Mol. Biol.

Cell

Biochimie

J. Struct. Biol.

Exp. Cell Res.

Cell

Exp. Cell Res.

J. Biol. Chem.

J. Mol. Biol.

Molecular aspects of the in vivo effects of ethionine, an analog of methionine

Microbiol. Rev.

cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera

Distinct molecular signals for nuclear import of the nucleolar snRNA, U3

Genes Dev.

Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA

EMBO J.

Secondary methylation of yeast ribosomal precursor RNA

Eur. J. Biochem.

RNA methylation and control of eukaryotic RNA biosynthesis

Eur. J. Biochem.

Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery

EMBO J.

Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization

Eur. J. Cell Biol.

Transcription-dependent colocalization of the U1, U2, U4U6 and U5 snRNPs in coiled bodies

J. Cell Biol.

Properties of ribosomes and ribosomal RNAs synthesized by Escherichia coli grown in the presence of ethionine

Eur. J. Biochem.

SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is is associated with the snR10 and snR11 small nuclear RNAs

J. Cell Biol.

RRP1, a Saccharomyces cerevisiae gene affecting rRNA processing and production of mature ribosomal subunits

J. Bacteriol.

GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast

EMBO J.

Depletion of U3 small nucleolar RNA inhibits cleavage in the 5′ external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA

EMBO J.

Article
Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly

Transcription-dependent colocalization of the U1, U2, $U 4 U6$ and U5 snRNPs in coiled bodies