Cell
ArticleMonoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm
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2005, Current Topics in Developmental BiologyCitation Excerpt :The various members encode additional motifs that likely have distinct functions during sperm activation, including one additional CRD in REJ1 (Moy et al., 1996), a single region of polycystic kidney disease (PKD) repeats and two transmembrane domains in REJ2 (Galindo et al., 2004), or a series of tandem PKD repeats and 11 transmembrane domains that form a cluster homologous to ion channels in REJ3 (Mengerink et al., 2002). Both REJ1 and REJ3 are cleaved at the GPS site, but the extracellular portion remains associated with the plasma membrane overlying the sperm acrosome (Mengerink et al., 2002; Moy et al., 1996; Trimmer et al., 1985). It is hypothesized that these ectodomains remain associated with the plasma membrane via heterogenic protein–protein interactions, likely anchored to the membrane via unmodified transmembrane REJ/PC members (Mengerink et al., 2002; Neill et al., 2004).
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2002, Journal of Biological ChemistryCitation Excerpt :Anti-BAF57 and anti-BAF57v were generated as follows: peptides corresponding to BAF57 (amino acids 394–411, ATVEEPPTDPIPEDEKKE) and BAF57v (amino acids 349–363, CPRKTLTSRYTDFPD) were synthesized, injected into rabbits, and used to prepare peptide affinity columns for antibody purification from antisera by ResGen (Invitrogen). Anti-CoREST monoclonal was generated following standard hybridoma methodology (34). The antibody was raised against GST fusion protein containing amino acids 101–293 of hCoREST.
Egg sialoglycans increase intracellular pH and potentiate the acrosome reaction of sea urchin sperm
2002, Journal of Biological ChemistryCitation Excerpt :Our previous studies (15, 16) showed that suREJ1 is a receptor for FSP. Antibodies showed that suREJ1 localizes to the entire flagellum as well as the acrosomal region (33). suREJ3, a homologue of suREJ1, localizes only to the acrosomal region (17).
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2000, Molecular Genetics and MetabolismNeuronal ER-plasma membrane junctions couple excitation to Ca<sup>2+</sup>-activated PKA signaling
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