Cell
Volume 40, Issue 3, March 1985, Pages 697-703
Journal home page for Cell

Article
Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

https://doi.org/10.1016/0092-8674(85)90218-1Get rights and content

Abstract

Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

References (40)

Cited by (79)

  • Defending the Zygote: Search for the Ancestral Animal Block to Polyspermy

    2005, Current Topics in Developmental Biology
    Citation Excerpt :

    The various members encode additional motifs that likely have distinct functions during sperm activation, including one additional CRD in REJ1 (Moy et al., 1996), a single region of polycystic kidney disease (PKD) repeats and two transmembrane domains in REJ2 (Galindo et al., 2004), or a series of tandem PKD repeats and 11 transmembrane domains that form a cluster homologous to ion channels in REJ3 (Mengerink et al., 2002). Both REJ1 and REJ3 are cleaved at the GPS site, but the extracellular portion remains associated with the plasma membrane overlying the sperm acrosome (Mengerink et al., 2002; Moy et al., 1996; Trimmer et al., 1985). It is hypothesized that these ectodomains remain associated with the plasma membrane via heterogenic protein–protein interactions, likely anchored to the membrane via unmodified transmembrane REJ/PC members (Mengerink et al., 2002; Neill et al., 2004).

  • Rest repression of neuronal genes requires components of the hSWI·SNF complex

    2002, Journal of Biological Chemistry
    Citation Excerpt :

    Anti-BAF57 and anti-BAF57v were generated as follows: peptides corresponding to BAF57 (amino acids 394–411, ATVEEPPTDPIPEDEKKE) and BAF57v (amino acids 349–363, CPRKTLTSRYTDFPD) were synthesized, injected into rabbits, and used to prepare peptide affinity columns for antibody purification from antisera by ResGen (Invitrogen). Anti-CoREST monoclonal was generated following standard hybridoma methodology (34). The antibody was raised against GST fusion protein containing amino acids 101–293 of hCoREST.

  • Egg sialoglycans increase intracellular pH and potentiate the acrosome reaction of sea urchin sperm

    2002, Journal of Biological Chemistry
    Citation Excerpt :

    Our previous studies (15, 16) showed that suREJ1 is a receptor for FSP. Antibodies showed that suREJ1 localizes to the entire flagellum as well as the acrosomal region (33). suREJ3, a homologue of suREJ1, localizes only to the acrosomal region (17).

View all citing articles on Scopus
View full text