Elsevier

Vision Research

Volume 23, Issue 11, 1983, Pages 1209-1216
Vision Research

Solitary horizontal cells in culture-I. Their electrical properties

https://doi.org/10.1016/0042-6989(83)90095-0Get rights and content

Abstract

Membrane properties of solitary horizontal cells were examined under current- and voltageclamp conditions. The resting membrane potential was mainly determined by K ions. The cells produced all-or-none action potentials and showed non-linear current-voltage relationships with inwardand outward-going rectification. Pharmacological and ion-substitution experiments under voltage-clamp conditions revealed that the ionic current was separated into at least four components; a Ca current and three types of K currents (a K current through an anomalous rectifier, a transient outward current, and a maintained outward current). The action potentials and the non-linear current-voltage relationships of solitary horizontal cells were explained from these ionic currents.

References (24)

  • ByzovA.L. et al.

    Amplification of graded potentials in horizontal cells of the retina

    Vision Res.

    (1977)
  • TrifonovY.A. et al.

    Electrical properties of subsynaptic and nonsynaptic membranes of horizontal cells in fish retina

    Brain res.

    (1974)
  • BaderC.R. et al.

    Responses to light of solitary rod photoreceptors isolated from tiger salamander retina

  • BaderC.R. et al.

    A voltage-clamp study of the light response in solitary rods of the tiger salamander

    J. Physiol.

    (1979)
  • BaylorD.A. et al.

    Receptive fields of cones in the retina of the turtle

    J. Physiol.

    (1971)
  • ByzovA.L. et al.

    The response to electric stimulation of horizontal cells in the carp retina

    Vision Res.

    (1963)
  • ConnorJ.A. et al.

    Voltage clamp studies of a transient outward membrane current in gastropod neural somata

    J. Physiol.

    (1971)
  • DowlingJ.E. et al.

    The interplexiform cell: a new tvpe of retinal neuron

    Invest. Ophthal.

    (1976)
  • DrujanB.D. et al.

    Characterization of different classes of isolated retinal cells

    Vision Res.

    (1972)
  • FainGL. et al.

    Membrane conductances of photoreceptors

    Prog. Biophys. Mol. Biol.

    (1981)
  • HagiwaraS. et al.

    Calcium channel. Ann. Rev, Neurosci.

    (1981)
  • HagiwaraS. et al.

    The anomalous rectification and cation selectivity of the membrane of a starfish egg cell

    J. membr. Biol.

    (1974)
  • Cited by (10)

    • Voltage- and calcium-gated ion channels of neurons in the vertebrate retina

      2019, Progress in Retinal and Eye Research
      Citation Excerpt :

      This is because of the low input resistance of horizontal cells that arises from strong gap junction coupling between horizontal cells and from the tonic activation of ionotropic glutamate receptors by glutamate released from photoreceptors (Aoyama et al., 2005; Miyachi and Murakami, 1989; Winslow and Ma, 1990). However, Na+ and Ca2+ -dependent action potentials are readily observed in solitary horizontal cells after enzymatic isolation (Blanco et al., 1996; Johnston and Lam, 1981; Shingai and Christensen, 1983; Tachibana, 1981, 1983b) and can be evoked in horizontal cells in vivo after inhibiting countervailing K+ currents (Murakami and Takahashi, 1987). The membrane potential of isolated horizontal cells typically shows two stable values: one at a negative value approaching EK and the other at a more positive value matching the plateau potential for action potentials.

    • Dynamic models of the retinal horizontal cell network

      1991, Progress in Biophysics and Molecular Biology
    View all citing articles on Scopus
    *

    Present address: Department of Information Physiology, National Institute for Physiological Sciences, Myodaijicho, Okazaki 444, Japan.

    View full text