Molecular spectroscopy in biodiagnostics (from Hippocrates to Herschel and beyond)
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Cited by (72)
Vibrational Spectroscopy Applications in Biomedical, Pharmaceutical and Food Sciences
2020, Vibrational Spectroscopy Applications in Biomedical, Pharmaceutical and Food SciencesConstruction of a two-in-one liposomal system (TWOLips) for tumor-targeted combination therapy
2014, International Journal of PharmaceuticsCitation Excerpt :We further compared TWOLips with liposomal vesicles and silica-coated liposomes alone using Fourier transform infrared (FTIR) spectroscopy and XPS studies. FTIR analysis (Fig. 3D–F) revealed that conventional liposomes displayed the characteristic CO peak of phospholipids at approximately 1740 cm−1 (Mantsch and Jackson, 1995). In contrast, silica-coated liposomes had peaks at 1080 cm−1 and 859 cm−1, which can be attributed to asymmetric SiOSi stretching and SiOSi bending vibrations, respectively (Li et al., 2012).
Lipid quantification method using FTIR spectroscopy applied on cancer cell extracts
2014, Biochimica et Biophysica Acta - Molecular and Cell Biology of LipidsCitation Excerpt :It was more surprising for vincristine. The difference spectrum for vincristine (Fig. 6B) displays a slight shift at the ν(CO) and a decrease of the phosphate vibrations (negative bands around 1240, 1085 and 970 cm− 1 [41,42]) relative to the ν(CO). A hypothesis that would account for these results is that the cells experience a change in the ratio between phospholipids and lipids containing no phosphate (fatty acids, triglycerides) which are not quantified by the approach proposed here.
The application of Fourier transform infrared microspectroscopy for the study of diseased central nervous system tissue
2012, NeuroImageCitation Excerpt :In lipid rich samples, the ester carbonyl band occurring at ~ 1725–1745 cm− 1 is prominent and minor bands at 1650 and 3010 cm− 1 (due to the C = C stretch and (C = C)H stretch, respectively) appear when unsaturated lipids are high (Christy and Egeberg, 2006). Absorption bands arising in the region between 1700 and 1500 cm− 1 are dominated by C = O and C―N stretching vibrations from amide groups, which comprise the peptide linkages in proteins (Byler and Susi, 1986; Mantsch and Jackson, 1995). In many biological samples, the amide I band is the most prominent band in the IR spectrum, arising from the C = O stretch with contributions from the N―H bending of proteins (Byler and Susi, 1986; Mantsch and Jackson, 1995).
Structure characterization of protein fractions from lotus (Nelumbo nucifera) seed
2011, Journal of Molecular StructureFTIR spectral signature of the effect of cardiotonic steroids with antitumoral properties on a prostate cancer cell line
2010, Biochimica et Biophysica Acta - Molecular Basis of DiseaseCitation Excerpt :More precisely, 19-hydroxy-hellebrin (Fig. 5D) still affected protein region i.e. amide I (1690–1620 cm− 1), amide II (1570–1530) [42,43] and various CH2/3 deformation mode arising from proteins (1468–1455 cm− 1) [42,45]. In addition to these areas, 19-hydroxy-2″-oxovoruscharin (Fig. 5B) also strongly modified the carbonyl associated with phospholipids (~ 1750–1700 cm− 1) [41,42] and regions associated with nucleic acids and glycosylations. As spectrum projection onto PC1 always showed the best separation between untreated and CS incubated cells (Fig. 5), the first principal components of each PCA were displayed on Fig. 6.