Journal of Molecular Biology
ArticleEnergy coupling in an ion pump: The reprotonation switch of bacteriorhodopsin☆
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A new group of eubacterial light-driven retinal-binding proton pumps with an unusual cytoplasmic proton donor
2015, Biochimica et Biophysica Acta - BioenergeticsChannelrhodopsin unchained: Structure and mechanism of a light-gated cation channel
2014, Biochimica et Biophysica Acta - BioenergeticsDynamic pictures of proteins by NMR
2014, Annual Reports on NMR SpectroscopyLight capture and energy transduction in bacterial rhodopsins and related proteins
2012, Comprehensive BiophysicsSuppressed or recovered intensities analysis in site-directed <sup>13</sup>C NMR: Assessment of low-frequency fluctuations in bacteriorhodopsin and D85N mutants revisited
2010, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :It was also shown that the peptide C = O of Val 49 forms a hydrogen bond with a water molecule connected to Asp 85 [70]. These observations are obviously the consequence of the fact that the tightly held helices are relaxed by loss of the electrostatic Schiff base–counter ion interaction [18] as a result of protonation to Asp 85 and deprotonation in the Schiff base [71–74]. The present conclusion is consistent with the previous observations, except for the abovementioned motions in the interhelical loops: low-resolution diffraction studies showed the characteristic conformational changes in the M-intermediate occur at helices B and G [15–17,67].
Mutagenic Analysis of Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example
2008, Methods in Cell Biology
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This work was funded by grants from the Department of Energy (DEFG03-86ER13525 to J.K.L. and DEFG0229498 to J.K.L.), the National Science Foundation (MCB-9202209 to R.N.), and the Ministry of Education, Science and Culture of Japan (905680579 to M.K.). The X-ray diffraction experiments were performed under approval by the Program Advisory Committee of the Photon Factory (Proposal 92-071).
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The authors wish to thank Drs T. Poulos for the use of the stopped-flow spectrophotometer and M. Ottolength for suggesting the use of the 13-trifluoromethyl retinal analog.