Journal of Molecular Biology
Two attached non-rigor crossbridge forms in insect flight muscle
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Cited by (33)
Regulation of Oscillatory Contraction in Insect Flight Muscle by Troponin
2010, Journal of Molecular BiologyInvertebrate muscles: Thin and thick filament structure; molecular basis of contraction and its regulation, catch and asynchronous muscle
2008, Progress in NeurobiologyCitation Excerpt :These changes were explained either as arising from a change in the nature, but not the number, of the cross-bridges (Barrington-Leigh et al., 1973; Goody et al., 1975, 1976; Beinbrech et al., 1976; Marston et al., 1976, 1979) or from a change in the activity of previously unattached cross-bridges (Wray, 1984). Later work showed that these interpretations were incorrect (Reedy et al., 1983a, 1987, 1988; Tregear et al., 1990; Biosca et al., 1990; Schmitz et al., 1996; Winkler et al., 1996). AMPPNP actually causes the release of the rear cross-bridges while inducing only small changes in shape and attachment angle of the lead ones (middle top and bottom panels, Fig. 7D).
Ca-activation and stretch-activation in insect flight muscle
2004, Biophysical JournalCitation Excerpt :Belostomatidae). The bundles were prepared from dorsal longitudinal muscles permeabilized in a protease-inhibitor cocktail by osmotic-shock treatment, cycling alternately between detergent and glycerol buffers before cryostorage in 75% glycerol relaxing buffer with 5 mM DTT at −80° to −100°C for up to one year before use, as described (Reedy et al., 1988). Fibers, selected for maximum transparency (only ∼30% develop during glycerination the glassy transparency that suits them for the striation follower), were moved from storing solution into relaxing solution.
Capturing time-resolved changes in molecular structure by negative staining
2003, Journal of Structural BiologyCitation Excerpt :In one, the lifetime of an intermediate can be prolonged, for example, by the stable binding of a chemical analog that mimics the intermediate state or of an inhibitor that arrests it. The structure is then observed by standard (non-time resolved) techniques (e.g., Applegate and Flicker, 1987; Avolio et al., 1984; Katayama, 1998; Reedy et al., 1988; Schmitz et al., 1997). The other approach involves rapid freezing of specimens at predetermined millisecond time intervals following initiation of a structural change.