General esterases of silkmoth moulting fluid: Preliminary characterization
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2022, Comparative Biochemistry and Physiology Part - C: Toxicology and PharmacologyCitation Excerpt :The increase in absorbance was measured spectrophotometrically at 340 nm for 5 min at 25 °C. ESTs were extracted and estimated using methodology of Katzenellenbogen and Kafatos (1971). Larval homogenate was prepared in chilled 0.1 M sodium phosphate buffer (pH 6.5).
Functional analysis of insect molting fluid proteins on the protection and regulation of ecdysis
2014, Journal of Biological ChemistryCitation Excerpt :Approximately 30 μg of cell lysates or 30 μg of molting fluid were loaded on each lane for native gel separation. Methods to detect superoxide dismutase (14), catalase (15), protease (16), and esterase (17) activities in a native gel were performed as described previously. To detect PO activity in molting fluids, each type of molting fluid (10 μg) was added directly to 100 μl of dopamine solution (10 mm dopamine dissolved in 10 mm Tris buffer, pH 7.5).
A tuber lectin from Arisaema helleborifolium Schott with anti-insect activity against melon fruit fly, Bactrocera cucurbitae (Coquillett) and anti-cancer effect on human cancer cell lines
2006, Archives of Biochemistry and BiophysicsCitation Excerpt :The larvae were then homogenized in the recommended buffer for each enzyme. The activity of esterases was determined by the method of Katzenellenbogen and Kafatos [26] while the activity of acid and alkaline phosphatases were assessed by the method given by Mc Intyre [27]. The results were expressed as means ± SE.
Physiology and Biochemistry of Insect Moulting Fluid
1996, Advances in Insect PhysiologyProperties of esterases from pharate pupal moulting fluid of the tobacco hornworm, Manduca sexta
1983, Comparative Biochemistry and Physiology -- Part B: Biochemistry andThe composition of larval-pupal moulting fluid in the tobacco hornworm, Manduca sexta
1978, Journal of Insect Physiology
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Present address: Department of Physiology-Biophysics, University of Illinois, Urbana, Illinois 61801.