Quantitative studies on brown algal phenols. I. Estimation of absolute polyphenol content of Ascophyllum nodosum (L.) Le Jol. and Fucus vesiculosus (L.)

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Abstract

Methods for estimating the absolute polyphenol content of the brown algae Ascophyllum nodosum (L.) Le Jol. and Fucus vesiculosus (L.) have been developed and tested. Polyphenols were extracted almost quantitatively from Ascophyllum nodosum using aqueous acetone, whereas this procedure was somewhat less efficient with Fucus vesiculosus. Colorimetric methods based on the Folin-Denis reagent, Brentamine Fast Red 2G Salt, and vanillin-H2SO4 were applied to acetone-free extracts for determination of polyphenol content relative to suitable reference compounds. Gravimetric methods based on hide powder and on haemoglobin were employed to derive ‘estimation factors’ (EFs) which allow calculation of the absolute polyphenol content from the relative polyphenol content. The values calculated for absolute polyphenol content are considered to be reasonably accurate, despite imprecisions in the methods and despite often large standard deviations, and re-emphasize the potential physiological and ecological significance of brown algal polyphenols. Although the precise EFs calculated here are not valid for other brown algae, the methods are considered to be generally applicable to other Phaeophyceae.

References (35)

  • Glombitza, K.-W., in press. Highly hydroxylated phenols from Phaeophyceae. In, Proceedings of the 8th international...
  • K.-W. Glombitza

    Highly hydroxylated phenols of the Phaeophyceae

  • A. Haug et al.

    Phenolic compounds in brown algae. I. The presence of reducing compounds in Ascophyllum nodosum (L.) Le Jol.

    Acta Chem. Scand.

    (1958)
  • A. Haug et al.

    Brunfarging av tangmel og tangekstrakter

    Norsk Inst. for Tang- og Tareforsk. Rapp.

    (1958)
  • A. Haug et al.

    Chemical composition of the brown alga Ascophyllum nodosum (L.) Le Jol.: influence of habitat on the chemical composition of Ascophyllum nodosum

    Nature, Lond.

    (1958)
  • G.V. Joshi et al.

    Seasonal variations in chemical composition of Sargassum ilicifolium Grun. and sea water

    Indian J. mar. Sci.

    (1975)
  • T. Katayama

    Tannins of seaweeds

    J. chem. Soc. Japan, Ind. Chem. Sect.

    (1951)
  • View full text