α,ω-Bis-quaternary ammonium alkanes as effective buffer additives for enhanced capillary electrophoretic separation of glycoproteins
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Non-covalent capillary coatings for protein separations in capillary electrophoresis
2008, Journal of Chromatography ACitation Excerpt :In CE, the adsorption of amines to the silanols on the capillary wall slows the EOF [36,37]. This slower EOF improves the resolution of anionic proteins where adsorption would not be expected, such as the separation of the glycoforms of ovalbumin [37,38]. To determine whether small amine additives prevent adsorption of cationic proteins, Verzola et al. performed systematic studies [39] in which the capillary was first equilibrated with FITC-labeled myoglobin at pH 5.0 (below myoglobin's pI of 7.6).
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