Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions

doi:10.1016/0016-5085(95)90564-2

Gastroenterology

Volume 109, Issue 4, October 1995, Pages 1068-1077

https://doi.org/10.1016/0016-5085(95)90564-2 Get rights and content

Abstract

$Background & Aims$ : The factors that stimulate the growth of gastric mucosal cells during gastric mucosal healing are not completely understood. This study was designed to investigate the gene expression of keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) in the healing of gastric mucosal lesions. $Methods$ : Northern blot analysis and reverse-transcription polymerase chain reaction were used to show HGF and KGF messenger RNA. $Results$ : Transcripts of HGF and KGF were not shown in rat gastric mucosal epithelium but were found in submucosal and muscular layers under normal conditions. When acute gastric mucosal lesions were induced by indomethacin treatment, expression of HGF messenger RNA was augmented in submucosal, muscular, or serosal layers, whereas the transcript of KGF was not influenced. When rat gastric mucosal epithelial cell line RGM1 and rat gastric mucosal primary culture cells were incubated with HGF or KGF, their proliferation was enhanced. $Conclusions$ : The results showed increased gene expression of HGF together with constant production of KGF during gastric mucosal healing.

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^☆: Supported by a grant-in-aid for scientific research from the Ministry of Education, Science, and Culture; a grant from the Ministry of Health and Welfare of Japan; and a fund of Cancer Research from Hyogo Total Health Association.

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Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions☆

Abstract

Gastroenterology

Biochem Biophys Res Commun

Biochem Biophys Res Commun

Biochem Biophys Res Commun

Biochem Biophys Res Commun

Gastroenterology

J Biol Chem

On cell proliferation and differentiation of fundic mucosa of the golden hamster

Cell Tissue Res

Tritiated thymidine autoradiographic study of cell migration and renewal in the pyloric mucosa of golden hamster

Cell Tissue Res

Tritiated thymidine autoradiographic study of cellular migration in the gastric gland of the golden hamster

Cell Tissue Res

Cell kinetics of human gastric mucosa and gastric cancer in organ culture

Virchows Arch

Deduced primary structure of rat hepatocyte growth factor and expression of the mRNA in rat tissues

Hepatocyte growth factor induces proliferation and morphogenesis in nonparenchymal epithelial liver cells

Hepatology

Purification and characterization of a newly identified growth factor specific for epithelial cells

Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth

Science

Clinical significance of human hepatocyte growth factor in blood from patients with fulminant hepatic failure

Hepatology