Histotypic self-organization by trypsin-dissociated and EDTA-dissociated chick embryo cells

doi:10.1016/0014-4827(68)90548-X

Experimental Cell Research

Volume 52, Issue 1, September 1968, Pages 71-85

https://doi.org/10.1016/0014-4827(68)90548-X Get rights and content

Abstract

The phenomenon of aggregation of chick embryo cells in vitro has been investigated by the rotation-mediated cell-aggregation procedure. In serum-supplemented media trypsin-dissociated neural-retina cells and EDTA-dissociated cells produced aggregates which appeared very similar in size and form. Drugs which are known to interfere with macromolecular synthesis, and which prevent the formation of rotation-mediated aggregates by trypsinized cells appear to be less effective in inhibiting the aggregation of EDTA dissociated cells. The aggregation of EDTA-dissociated cells does not appear to be affected by periodate or by borate at concentrations that significantly inhibit the aggregation of trypsin-dissociated cells. The aggregation of both EDTA-dissociated and trypsin-dissociated retina and liver cells is greatly inhibited by 0.5 per cent glucosamine; however, numerous other aminosugars and other organic amines will cause a similar inhibition at concentrations of 0.5 per cent or less.

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^☆: This work has been supported by funds from the United States Atomic Energy Commission, the United States Public Health Service, and the American Heart Association.

¹: Present address: Department of Biochemistry, South Parks Road, Oxford University, Oxford, UK.

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Histotypic self-organization by trypsin-dissociated and EDTA-dissociated chick embryo cells☆

Abstract

Develop. Biol

Exptl Cell Res

Exptl Cell Res

Exptl Cell Res

Science

Exptl Cell Res

Advances in Enzymol

Exptl Cell Res

Nature

J. Embryol. Exptl Morphol

Science

Radiation Res