Somatostatin and analogs inhibit endogenous synaptic plasma membrane protein phosphorylation in vitro

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Abstract

The addition of somatostatin to hippocampal synaptic plasma membrane (SPM) preparations in vitro decreased subsequent phosphorylation of specific protein bands. 10−4 M somatostatin inhibited the phosphorylation of protein bands with apparent molecular weights between 10 000 and 20 000 daltons and, to a lesser extent, 48 000 daltons (B-50) and 52 000. Increasingly greater degrees of inhibition were seen in response to somatostatin-28 and [D-Trp8]somatostatin. Inhibition of B-50 protein phosphorylation in the presence of [D-Trp8]somatostatin was most prominent in SPM preparations from the hippocampus and amygdala, with lesser degrees of inhibition seen in the cortex and hypothalamus. Addition of [D-Trp8]somatostatin to an ammonium sulfate-precipitated fraction (ASP 55–80) from cortex only slightly inhibited endogenous B-50 phosphorylation. The injection of [D-Trp8] somatostatin intracerebroventricularly into rats did not induce excessive grooming behavior but resulted in barrel rotation. These results suggest that somatostatin and congeners affect SPM protein phosphorylation in a manner different from that of ACTH, presumably involving membrane sites that bind somatostatin.

References (36)

  • J.-C. Reubi et al.

    High affinity binding sites for a somatostatin-28 analog in rat brain

    Life Sci.

    (1981)
  • M. Rezek et al.

    Central site of action of somatostatin (SRIF): role of hippocampus

    Neuropharmacol.

    (1976)
  • R.G. Sorensen et al.

    Presynaptic localization of phosphoprotein B-50

    Brain Res. Bull.

    (1981)
  • M. Williams et al.

    Protein phosphorylation in nervous tissue: Possible involvement in nervous tissue function and relationship to cyclic nucleotide metabolism

    Progr. Neurobiol.

    (1977)
  • H. Zwiers et al.

    Behavioral and neurochemical effects of the new opioid peptide dynorphin-(1–13): Comparison with other neuropeptides

    Life Sci.

    (1981)
  • J.H. Brakkee et al.

    A simple technique for rapid implantation of a permanent cannula into the rat brain ventricular system

    Laboratory Animal Sci.

    (1979)
  • P. Davies et al.

    Reduced somatostatin-like immunoreactivity in cerebral cortex from cases of Alzheimer disease and Alzheimer senile dementia

    Nature

    (1980)
  • E. DeRobertis et al.

    Structural components of the synaptic region

  • Cited by (0)

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