Elsevier

Developmental Biology

Volume 61, Issue 1, November 1977, Pages 20-27
Developmental Biology

Full paper
Interaction of peanut agglutinin, a lectin specific for nonreducing terminal d-galactosyl residues, with embryonal carcinoma cells

https://doi.org/10.1016/0012-1606(77)90338-4Get rights and content

Abstract

Peanut agglutinin (PNA), a lectin specific for terminal d-galactosyl residues, was found to react with embryonal carcinoma cells, but not with their differentiated derivatives. Receptors for PNA were detectable at the surface of all cells of the quasinullipotent F9 line and on only 50% of the multipotent PCC3/A/1 line. The fraction of the PCC3/A/1 population which expresses the F9 antigen was found to be included in the subpopulation carrying the PNA receptors. PNA+ and PNA subpopulations of PCC3/A/1 were separated by a PNA-mediated reversible agglutination of PNA+ cells with rabbit erythrocytes. These subpopulations were essentially F9+ and F9, respectively.

References (27)

Cited by (99)

  • Activity of N-acylneuraminate-9-phosphatase (NANP) is not essential for de novo sialic acid biosynthesis

    2019, Biochimica et Biophysica Acta - General Subjects
    Citation Excerpt :

    We used flow cytometry to quantify binding with two additional lectins. Maackia amurensis Lectin II (MAL-II) recognizes α2,3 linked sialic acid in O-glycans [19] and peanut agglutinin (PNA) was used to probe exposed β1,3 galactosyl groups to show desialylation of O-glycans [20]. Incubation of CHO KO cells with MAL-II and PNA lectins followed by fluorescent labelling and analysis with a spectral analyzer showed decreased binding to CHO cells with KO of gne, nans and cmas compared to wt cells (Fig. 1C).

  • Nathan Sharon. 1925-2011

    2012, Advances in Carbohydrate Chemistry and Biochemistry
View all citing articles on Scopus
View full text