Estimation of VLDL cholesterol in hyperlipidemia
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Association of the lipoprotein lipase and Apolipoprotein C-II gene polymorphisms with risk of dyslipidemia in smokers and non-smokers male
2022, Indian Heart JournalCitation Excerpt :Analyses of the lipid profiles were performed on the study population 185 after fasting for at least 12 h before the collection of blood samples by venepuncture. Conventional enzymatic-colourimetric (Biolabo/France) methods were used to determine the serum concentrations of TG, TC and HDL-C. VLDL-C was calculated by dividing the TG by 2.2 (units mmol/L), estimated VLDL-C can be used along with quantitation of HDL-C and TC using the Friedewald equation to calculate LDL-C.9LDL-C (mmol/l) = TC- HDL-C - (TG)/2.2 …. 10 Genomic DNA was isolated from fresh or frozen whole blood cells in EDTA tubes using the Geneaid DNA purification kit and achieved according to the method recommended by the manufacturing company Geneaid (Geneaid/USA).
Evaluation of four different equations for calculating LDL-C with eight different direct HDL-C assays
2013, Clinica Chimica ActaCitation Excerpt :Most of the direct HDL-C assays begin to show errors in samples with TG > 200 mg/dl [10,11], and hence this cut point was used in this study but comparing the cLDL-C equations at a lower TG thresholds may reveal an advantage for some equations for over the others. Another limitation of this study is that other equations besides the ones tested have been described for estimating LDL-C [17,18,21], but we have tried to focus here on the more recent and more popularly used cLDL-C equations. Finally, the samples collected for this study were largely obtained from patients with dyslipidemia and cardiovascular disease and may not be representative of the general population.
The effect of acute café latte ingestion on fasting serum lipid levels in healthy individuals
2013, Journal of Clinical LipidologyPolymorphism at the TRIB1 gene modulates plasma lipid levels: Insight from the Spanish familial hypercholesterolemia cohort study
2011, Nutrition, Metabolism and Cardiovascular DiseasesCitation Excerpt :HDL-C was determined after precipitation with phosphotungstate [20]. Very low-density lipoprotein cholesterol (VLDL-C) was determined from plasma TG [21]. ApoA-I and B levels were determined by immunoturbidimetry [22].
Genetic variations at ABCG5/G8 genes modulate plasma lipids concentrations in patients with familial hypercholesterolemia
2010, AtherosclerosisCitation Excerpt :Total cholesterol (TC) and TG were determined by enzymatic techniques [16,17]. Very low-density lipoprotein cholesterol (VLDL-C) was determined from plasma TG [18]. HDL-C was determined after precipitation with phosphotungstate [19].