Selective loss of neurofilament proteins after exposure of differentiated human IMR-32 neuroblastoma cells to oxidative stress

doi:10.1016/0006-8993(96)00992-4

Brain Research

Volume 738, Issue 1, 28 October 1996, Pages 162-166

https://doi.org/10.1016/0006-8993(96)00992-4 Get rights and content

Abstract

Millimolar concentrations of ascorbate in the presence of iron can cause neuronal cell death. This study shows that the human neuronal cell line IMR-32 is sensitive to ascorbate and that cytotoxicity can be blocked by the antioxidant enzymes Cu/Zn-superoxide dismutase and catalase. There was a selective loss of neurofilament proteins after exposure to 5 or 10 mM ascorbate, as assessed by immunostaining and by Western blotting. Loss of actin or tubulin was not seen, suggesting that loss of neurofilaments is a sensitive and selective marker for free radical damage in these cells.

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Acknowledgements

The authors would like to thank Mr. David Hughes and Ms. Debbie Jones for their assistance with the IMR-32 cell cultures and Mr. Arthur Oakley for his photographic assistance. P.J.S. is supported by the Wellcome Trust as a Senior Fellow in Clinical Science.

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Short communicationSelective loss of neurofilament proteins after exposure of differentiated human IMR-32 neuroblastoma cells to oxidative stress

Abstract

Section snippets

Acknowledgements

Neurosci. Lett.

Int. J. Biochem. Cell Biol.

J. Neurol. Sci.

Trends Neurosci.

Trends Neurol. Sci.

Free Rad. Biol. Med.

Neurofilaments, free radicals and amyotrophic lateral sclerosis

Muscle and Nerve

Superoxide dismutase activity, oxidative damage, and mitochondrial energy metabolism in familial and sporadic amyotrophic lateral sclerosis

J. Neurochem.

Short communication
Selective loss of neurofilament proteins after exposure of differentiated human IMR-32 neuroblastoma cells to oxidative stress